Recombinant Rabbit Anti-NR1H3 Antibody (clone R07-6A4)
CAT#: VS3-FY1065
This product is a recombinant rabbit antibody that recognizes NR1H3. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin. The antigen is synthetic peptide of human LXRα. The clone R07-6A4 is specific for human, rat NR1H3.
Tested Data
Gene Expression
WB
Figure 1. Recombinant Rabbit Anti-NR1H3 Antibody (clone R07-6A4) in WB.
Western Blot analysis of LXR α in rat brain lysates using LXR α Antibody.
IHC-P
Figure 2. Recombinant Rabbit Anti-NR1H3 Antibody (clone R07-6A4) in IHC-P.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using LXR α antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Subcellular Location
Figure 1 IF staining of human cell line HeLa
Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36443/1050_C11_2_selected.jpg
Normal Tissue
Figure 2 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37109/94492_A_8_8.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37109/94492_A_8_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37109/94492_A_9_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37109/94492_A_4_6.jpg
Normal Tissue
Figure 6 Placenta
Decidual cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Trophoblastic cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37109/94492_A_1_7.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (ASC TERT1, HSkMC)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000025434-NR1H3
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Specifications
- Immunogen
- Synthetic peptide of human LXRα.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Rat NR1H3
- Species Reactivity
- Human, Rat
- Clone
- R07-6A4
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
- MW
- Calculated MW: 50 kDa; Observed MW: 50 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "NR1H3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2318CT | Recombinant Mouse anti-Human NR1H3 Monoclonal antibody (FQS3403) | ChIP, WB, ELISA, IP, GSA, IHC-P, FC | |
| MRO-0946-CN | Recombinant Rabbit Anti-NR1H3 Monoclonal Antibody (JA20-38) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-WK239 | Rabbit Anti-NR1H3 Recombinant Antibody (clone JA20-38) | WB, ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2488 | Hi-Affi™ Recombinant Rabbit Anti-NR1H3 Monoclonal Antibody (DS2488AB) | FC, WB, ICC, IF | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC837 | CytoStream™ Rabbit Anti-NR1H3 Recombinant Antibody (VS13-YC837) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1512 | Anti-NR1H3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4943 | Anti-Mouse NR1H3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4942 | Anti-Human NR1H3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4944 | Anti-Rat NR1H3 Immunohistochemistry Kit | IHC |
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