Recombinant Rabbit Anti-NR1H3 Antibody (clone R07-6A4) (CAT#: VS3-FY1065)

Name: Recombinant Rabbit Anti-NR1H3 Antibody (clone R07-6A4)
SKU: VS3-FY1065
Rating: 5 (1 reviews)

This product is a recombinant rabbit antibody that recognizes NR1H3. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin. The antigen is synthetic peptide of human LXRα. The clone R07-6A4 is specific for human, rat NR1H3.

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Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1. Recombinant Rabbit Anti-NR1H3 Antibody (clone R07-6A4) in WB.

Figure 1. Recombinant Rabbit Anti-NR1H3 Antibody (clone R07-6A4) in WB.

Western Blot analysis of LXR α in rat brain lysates using LXR α Antibody.

IHC-P

Figure 2. Recombinant Rabbit Anti-NR1H3 Antibody (clone R07-6A4) in IHC-P.

Figure 2. Recombinant Rabbit Anti-NR1H3 Antibody (clone R07-6A4) in IHC-P.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using LXR α antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.

Subcellular Location

Figure 1 IF staining of human cell line HeLa
(Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36443/1050_C11_2_selected.jpg

Normal Tissue

Figure 2 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37109/94492_A_8_8.jpg

Figure 3 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37109/94492_A_8_3.jpg

Figure 4 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37109/94492_A_9_5.jpg

Figure 5 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37109/94492_A_4_6.jpg

Figure 6 Placenta
(Decidual cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Trophoblastic cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37109/94492_A_1_7.jpg

RNA Expression

Figure 7 RNA cell line category: Cell line enhanced (ASC TERT1, HSkMC)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000025434-NR1H3

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Synthetic peptide of human LXRα.

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human, Rat NR1H3

Species Reactivity
Human, Rat

Clone
R07-6A4

Applications
Western Blot, Immunohistochemistry-Paraffin

Conjugate
Unconjugated

MW
Calculated MW: 50 kDa; Observed MW: 50 kDa

Product Property

Purification
Affinity Purified

Purity
>95% as determined by SDS-PAGE

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100

Target

Alternative Names
Nuclear Receptor Subfamily 1 Group H Member 3; LXRA; Nuclear Receptor Subfamily 1, Group H, Member 3; Liver X Nuclear Receptor Alpha Variant 1; Oxysterols Receptor LXR-Alpha; Liver X Receptor-Alpha; Liver X Receptor Alpha; LXR-A; RLD-1

Gene ID
10062

UniProt ID
Q13133

Secondary Antibody

Isotype Control

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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IgG Antibody Products Rabbit Monoclonal Antibody Products Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry

CAT	Product Name	Application	Type
MOB-2318CT	Recombinant Mouse anti-Human NR1H3 Monoclonal antibody (FQS3403)	ChIP, WB, ELISA, IP, GSA, IHC-P, FC
MRO-0946-CN	Recombinant Rabbit Anti-NR1H3 Monoclonal Antibody (JA20-38)	WB, IF, IHC, FC	Rabbit IgG
VS3-WK239	Rabbit Anti-NR1H3 Recombinant Antibody (clone JA20-38)	WB, ELISA	Rabbit IgG

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