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    Recombinant Anti-PDCD1LG2 Vesicular Antibody, EV Displayed (VS-0425-YC279) (CAT#: VS-0425-YC279)

    The Recombinant Anti-PDCD1LG2 Vesicular Antibody, EV Displayed (VS-0425-YC279) is an antibody-displaying extracellular vesicle (Ab-EV). The product combines the benefits of both extracellular vesicle (EV) and antibody (Ab) which can guide the decorated EVs to PDCD1LG2-expressed cells or tissues. The PDCD1LG2 is involved in negatively regulating T cell proliferation and interleukin-10 production, with implications in tuberculosis.

    Specific Inquiry
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line U-2 OS
    Figure 1 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/104_F6_1_selected.jpg
    Figure 2 IHC staining of human pancreas
    Figure 2 IHC staining of human pancreas
    (Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity with granular pattern in exocrine glandular cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/ihc_selected.jpg
    Figure 3 IF staining of human cell line U-251 MG
    Figure 3 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/200_H4_1_blue_red_green.jpg
    Normal Tissue
    Figure 4 Cerebral cortex
    Figure 4 Cerebral cortex
    (Neuropil
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/30825_B_9_5.jpg
    Figure 5 Colon
    Figure 5 Colon
    (Glandular cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/30825_A_9_3.jpg
    Figure 6 Liver
    Figure 6 Liver
    (Cholangiocytes
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Hepatocytes
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/30825_A_8_4.jpg
    Figure 7 Pancreas
    Figure 7 Pancreas
    (Exocrine glandular cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Pancreatic endocrine cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/30825_A_2_3.jpg
    Figure 8 Kidney
    Figure 8 Kidney
    (Cells in tubules
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/30825_A_9_5.jpg
    Figure 9 Testis
    Figure 9 Testis
    (Leydig cells
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/30825_A_6_6.jpg
    Figure 10 Lymph node
    Figure 10 Lymph node
    (Germinal center cells
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous
    Non-germinal center cells
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/30825_A_9_8.jpg
    RNA Expression
    Figure 11 RNA cell line category: Cell line enhanced (BJ, BJ hTERT+, HDLM-2, TIME
    Figure 11 RNA cell line category: Cell line enhanced (BJ, BJ hTERT+, HDLM-2, TIME
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000197646-PDCD1LG2

    Recombinant Antibody

    • Application
    • ELISA, FC, Cell-uptake
    • Product Type
    • Ab-Fc-EVs
    • Antibody Quantification (Ab/EV)
    • ~100 Ab/EV
    • Target
    • PDCD1LG2
    • Host Animal
    • Mouse
    • Antibody Isotype
    • IgG
    • Species Reactivity
    • Human
    • Expression Cell
    • Mammalian cell

    Engineered EVs

    • EV-sorting domain
    • CD63
    • Fc-binding domain
    • Protein A
    • EV Size
    • 30~150 nm
    • Producing Cell
    • HEK293F
    • Isolation Method
    • Gradient centrifugation
    • Purification
    • qEV size exclusion chromatography
    • Concentration
    • 1 x 10¹⁰
    • Size
    • 1 mL
    • Buffer
    • PBS
    • Storage
    • Store at -80°C for 12 months

    Target

    • Full Name
    • Programmed cell death 1 ligand 2
    • Biological Process
    • Adaptive immunity, Immunity
    • Molecular Function
    • Receptor
    • Cellular Localization
    • Cytosol
    • Introduction
    • Involved in negative regulation of activated T cell proliferation; negative regulation of interleukin-10 production; and negative regulation of type II interferon production. Predicted to be located in plasma membrane. Predicted to be active in external side of plasma membrane. Biomarker of pulmonary tuberculosis.
    • Alternative Names
    • B7-DC, bA574F11.2, Btdc, CD273, PD-L2, PDL2

    Downloads

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    CAT Product Name Application Type
    NAB-993-VHH Recombinant Anti-Mouse Pdcd1lg2 VHH Single Domain Antibody WB, ICC, ChiP, FA, ELISA Llama VHH

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