Recombinant Mouse Anti-PDHA1 Antibody (VS4-CJ70)
CAT#: VS4-CJ70
This mouse anti-PDHA1 antibody is a product that recognizes human, dog, rat, mouse, porcine PDHA1. The immunogen of this antibody is recombinant fragment, corresponding to amino acids 131aa-389aa of human PDHA1 (C-terminus). The mAb has been tested for use in ELISA, Western Blot (1:500-1:5000), Immunocytochemistry (1:50-1:200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HeLa
Immunofluorescent staining of human cell line HeLa shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47864/1645_G11_19_cr57b568f223f4d_selected.jpg
Normal Tissue
Figure 2 Heart muscle
Cardiomyocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47864/113115_B_5_6.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47487/113196_A_9_8.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47864/113115_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47864/113115_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47864/113115_A_6_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000131828-PDHA1
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Specifications
- Immunogen
- Recombinant fragment, corresponding to amino acids 131aa-389aa of human PDHA1 (C-terminus).
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human, Dog, Rat, Mouse, Porcine PDHA1
- Species Reactivity
- Human, Dog, Rat, Mouse, Porcine
- Applications
- ELISA, WB, ICC
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.1M Tris-Glycine (150 mM NaCl), pH 7.4.
- Preservative
- 0.2% Sodium Azide
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Western Blot (1:500-1:5000), Immunocytochemistry (1:50-1:200).
Target
- Alternative Names
- PDHA; PDHAD; PHE1A; E1alpha; PDHCE1A
- Gene ID
- 5160
- UniProt ID
- P08559
- Long Name
- Pyruvate dehydrogenase E1 subunit alpha 1
- Cellular Localization
- Mitochondrion
- Post Translation Modifications
- Phosphorylation at Ser-232, Ser-293 and Ser-300 by PDK family kinases inactivates the enzyme; for this phosphorylation at a single site is sufficient. Dephosphorylation at all three sites, i.e. at Ser-232, Ser-293 and Ser-300, is required for reactivation.
Acetylation alters the phosphorylation pattern. Deacetylated by SIRT3 (By similarity).
- Protein Refseq
- NP_000275.1; NP_001166925.1; NP_001166926.1
- Function
- The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2, and thereby links the glycolytic pathway to the tricarboxylic cycle.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PDHA1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2639 | Hi-Affi™ Recombinant Rabbit Anti-PDHA1 Monoclonal Antibody (DS2639AB) | WB, IHC-P, ICC, IF, IP, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC4342 | PE-A*01:01/Human PDHA1 (KSDPIMLLK) MHC Tetramer | FCM | |
| MHC-LC4343 | PE-A*03:01/Human PDHA1 (KSDPIMLLK) MHC Tetramer | FCM | |
| MHC-LC4344 | PE-A*11:01/Human PDHA1 (KSDPIMLLK) MHC Tetramer | FCM | |
| MHC-LC4345 | PE-A*69:01/Human PDHA1 (KSDPIMLLK) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0364C | Mouse Anti-PDHA1 Recombinant Antibody (ZG-0364C) | WB, IF | Mouse IgG |
| VS3-WK441 | Rabbit Anti-PDHA1 Recombinant Antibody (clone JF996-0) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| VS3-FY1115 | Recombinant Mouse Anti-PDHA1 Antibody (clone 1G10C10) | ELISA, WB, IP, IHC, ICC, FC | Mouse IgG1 |
| VS3-FY1116 | Recombinant Mouse Anti-PDHA1 Antibody (clone 2A2H7) | ELISA, WB, FC | Mouse IgG2b |
| VS3-FY1117 | Recombinant Mouse Anti-PDHA1 Antibody (clone 4E12D6) | ELISA, WB, IHC, ICC, FC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY372 | Rabbit Anti-NHP PDHA1 Recombinant Antibody (VS-1024-XY372) | WB, IHC, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1601 | Anti-PDHA1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5281 | Anti-Mouse PDHA1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5280 | Anti-Human PDHA1 Immunohistochemistry Kit | IHC |
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