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Recombinant Rabbit Anti-PDK2 Antibody (clone R01-6D8)

Recombinant Rabbit Anti-PDK2 Antibody (clone R01-6D8) (CAT#: VS3-FY1120)

Name: Recombinant Rabbit Anti-PDK2 Antibody (clone R01-6D8)
SKU: VS3-FY1120
Rating: 5 (1 reviews)

This product is a recombinant rabbit antibody that recognizes PDK2. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation. The clone R01-6D8 is specific for human, mouse, rat PDK2. The antigen is synthetic peptide of human pdk2.

Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1. Recombinant Rabbit Anti-PDK2 Antibody (clone R01-6D8) in WB.

Figure 1. Recombinant Rabbit Anti-PDK2 Antibody (clone R01-6D8) in WB.

Western Blot analysis of PDK2 in rat brain lysates using a PDK2 antibody.

Subcellular Location

Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8287/41_C4_2_selected.jpg

Normal Tissue

Figure 2 IHC staining of human heart muscle
(Immunohistochemical staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8287/ihc_selected.jpg

Figure 3 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8287/21991_B_7_5.jpg

Figure 4 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8287/21991_A_9_3.jpg

Figure 5 Testis
(Elongated or late spermatids Staining: Low Intensity: Moderate Quantity: <25% Leydig cells Staining: Medium Intensity: Strong Quantity: <25% Pachytene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8287/21991_A_5_6.jpg

Figure 6 Skeletal muscle
(Myocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8287/21991_B_3_6.jpg

Figure 7 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8287/21991_A_8_8.jpg

RNA Expression

Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000005882-PDK2

Datasheet

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Synthetic peptide of human PDK2.

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human, Mouse, Rat PDK2

Species Reactivity
Human, Mouse, Rat

Clone
R01-6D8

Applications
Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation

Conjugate
Unconjugated

MW
Calculated MW: 46 kDa; Observed MW: 46 kDa

Product Property

Purification
Affinity Purified

Purity
>95% as determined by SDS-PAGE

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunoprecipitation: 1/20

Target

Alternative Names
Pyruvate Dehydrogenase Kinase 2; Pyruvate Dehydrogenase Kinase, Isoenzyme 2; Pyruvate Dehydrogenase Kinase, Isozyme 2; PDH Kinase 2; EC 2.7.11.2; PDHK2

Gene ID
5164

UniProt ID
Q15119

Secondary Antibody

Isotype Control

Related Resources

Related Signaling Pathways

PPAR Signaling Pathway

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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IgG Antibody Products Rabbit Monoclonal Antibody Products

CAT	Product Name	Application	Type
MOB-1050MZ	Recombinant Mouse Anti-Human PDK2 Antibody	WB	Mouse antibody
MRO-1199-CN	Recombinant Rabbit Anti-PDK2 Monoclonal Antibody (JB66-93)	WB, IHC, FC, IP	Rabbit IgG
VS3-WK323	Rabbit Anti-PDK2 Recombinant Antibody (clone JB66-93)	WB, ELISA	Rabbit IgG
VS3-XY1233	Mouse Anti-PDK2 Recombinant Antibody (clone 3F2C4)	IHC, ICC, FC	Mouse IgG2b
VS3-XY3460	Rabbit Anti-PDK2 Recombinant Antibody (clone R01-5X9)	WB, IHC-P, ICC, IF, IP, FC	Rabbit IgG

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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