Recombinant Rabbit Anti-PDK2 Antibody (clone R01-6D8)
CAT#: VS3-FY1120
This product is a recombinant rabbit antibody that recognizes PDK2. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation. The clone R01-6D8 is specific for human, mouse, rat PDK2. The antigen is synthetic peptide of human pdk2.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8287/41_C4_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human heart muscle
Immunohistochemical staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8287/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8287/21991_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8287/21991_A_9_3.jpg
Normal Tissue
Figure 5 Testis
Elongated or late spermatids Staining: Low Intensity: Moderate Quantity: <25% Leydig cells Staining: Medium Intensity: Strong Quantity: <25% Pachytene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8287/21991_A_5_6.jpg
Normal Tissue
Figure 6 Skeletal muscle
Myocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8287/21991_B_3_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8287/21991_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000005882-PDK2
❮
❯
WB
Figure 1. Recombinant Rabbit Anti-PDK2 Antibody (clone R01-6D8) in WB.
Western Blot analysis of PDK2 in rat brain lysates using a PDK2 antibody.
❮
❯
Specifications
- Immunogen
- Synthetic peptide of human PDK2.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat PDK2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-6D8
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 46 kDa; Observed MW: 46 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunoprecipitation: 1/20
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-FY1120. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Related Signaling Pathways
PPAR Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "PDK2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1050MZ | Recombinant Mouse Anti-Human PDK2 Antibody | WB | Mouse antibody |
| MRO-1199-CN | Recombinant Rabbit Anti-PDK2 Monoclonal Antibody (JB66-93) | WB, IHC, FC, IP | Rabbit IgG |
| VS3-QX862 | Mouse Anti-PDK2 Recombinant Antibody (clone 2B8-C8-F6) | WB, IHC, ICC, FC, ELISA | Mouse IgG2b |
| VS3-QX863 | Mouse Anti-PDK2 Recombinant Antibody (clone 180CT10.2.3) | WB, IHC | Mouse IgG1, κ |
| VS3-XY1233 | Mouse Anti-PDK2 Recombinant Antibody (clone 3F2C4) | IHC, ICC, FC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2645 | Hi-Affi™ Recombinant Rabbit Anti-PDK2 Monoclonal Antibody (DS2645AB) | FC, ICC, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1607 | Anti-PDK2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5292 | Anti-Mouse PDK2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5291 | Anti-Human PDK2 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: WB, ELISA, IF
Application: ELISA, FC, Inhib, FuncS
Application: ELISA, Neut
Application: FC
-2.png)
Application: WB, ELISA, FuncS
Application: EM, ELISA, ICC, IHC-Fr, IHC-P, WB
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
