Recombinant Mouse Anti-PGR Antibody (clone 2E4B9) (CAT#: VS3-FY1132)

• Host Species
• Mouse

• Type
• Mouse IgG1

• Clone
• 2E4B9

• Applications
• Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry, Immunohistochemistry

• Conjugate
• Unconjugated

• Purity
• >95% as determined by SDS-PAGE

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry, Immunohistochemistry.

• Alternative Names
• Progesterone Receptor; Nuclear Receptor Subfamily 3 Group C Member 3; NR3C3; PR

• Gene ID
• 5241

• UniProt ID
• P06401

• Sequence Similarities
• Belongs to the nuclear hormone receptor family. NR3 subfamily.

• Cellular Localization
• Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus

• Post Translation Modifications
• Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 ^oCcurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G2/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.
  Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.

• Protein Refseq
• NP_000917.3; NP_001189403.1; NP_001258090.1

• Function
• The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Depending on the isoform, progesterone receptor functions as transcriptional activator or repressor.
  Isoform A: Ligand-dependent transdominant repressor of steroid hormone receptor transcriptional activity including repression of its isoform B, MR and ER. Transrepressional activity may involve recruitment of corepressor NCOR2.
  Isoform B: Transcriptional activator of several progesteron-dependent promoters in a variety of cell types. Involved in activation of SRC-dependent MAPK signaling on hormone stimulation.
  Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.

Secondary Antibody

• 2ND-047CQ
• Goat Anti-Mouse IgG1 Antibody, R-PE

• 2RD-A1460-YJ
• Goat Anti-Mouse IgG1 Secondary antibody

Isotype Control

• MOB-065CQ
• Mouse IgG1 Isotype Control Antibody