Recombinant Mouse Anti-PGR Antibody (clone 5C10-6H2-6B1)
CAT#: VS3-FY1134
This product is a recombinant mouse antibody that recognizes PGR. This antibody has been reported for use in Immunohistochemistry-Paraffin. The clone 5C10-6H2-6B1 is specific for human PGR. The antigen is synthetic peptide derived from human progesterone receptor (pr).
Gene Expression
Tested Data
Normal Tissue
Figure 1 Fallopian tube
Glandular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8428/21817_A_8_7.jpg
RNA Expression
Figure 2 RNA cell line category: Cell line enriched (T-47d)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000082175-PGR
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IHC-P
Figure 1. Recombinant Mouse Anti-PGR Antibody (clone 5C10-6H2-6B1) in IHC-P.
Immunohistochemical analysis of paraffin-embedded breast carcinoma using progesterone receptor antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
IHC-P
Figure 2. Recombinant Mouse Anti-PGR Antibody (clone 5C10-6H2-6B1) in IHC-P.
Immunohistochemical analysis of paraffin-embedded breast carcinoma using progesterone receptor antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- Synthetic peptide derived from human progesterone receptor (PR).
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human PGR
- Species Reactivity
- Human
- Clone
- 5C10-6H2-6B1
- Applications
- Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.3, 50% glycerol, 0.5% BSA
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry-Paraffin: 1/100-1/500
Target
- Alternative Names
- Progesterone Receptor; Nuclear Receptor Subfamily 3 Group C Member 3; NR3C3; PR
- Gene ID
- 5241
- UniProt ID
- P06401
- Sequence Similarities
- Belongs to the nuclear hormone receptor family. NR3 subfamily.
- Cellular Localization
- Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus
- Post Translation Modifications
- Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 oCcurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G2/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.
Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.
- Protein Refseq
- NP_000917.3; NP_001189403.1; NP_001258090.1
- Function
- The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Depending on the isoform, progesterone receptor functions as transcriptional activator or repressor.
Isoform A: Ligand-dependent transdominant repressor of steroid hormone receptor transcriptional activity including repression of its isoform B, MR and ER. Transrepressional activity may involve recruitment of corepressor NCOR2.
Isoform B: Transcriptional activator of several progesteron-dependent promoters in a variety of cell types. Involved in activation of SRC-dependent MAPK signaling on hormone stimulation.
Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
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Reconstitution Buffer
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5355 | Anti-PGR Immunohistochemistry Kit | IHC | |
| VS-0525-XY5356 | Anti-Human PGR Immunohistochemistry Kit | IHC | |
| VS-0525-XY5357 | Anti-Mouse PGR Immunohistochemistry Kit | IHC |
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