Recombinant Mouse Anti-PIK3CA Antibody (clone 3C1-6F9-6C4)
CAT#: VS3-FY1138
This product is a recombinant mouse antibody that recognizes PIK3CA. This antibody has been reported for use in Immunohistochemistry-Paraffin. The clone 3C1-6F9-6C4 is specific for human PIK3CA. The antigen is synthetic peptide derived from human pik3ca h1047r.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/68_B8_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human adrenal gland
Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in cortical cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human appendix
Immunohistochemical staining of human appendix shows cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17804/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/24769_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/24713_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/24713_A_9_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/24713_A_9_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/24713_A_6_6.jpg
Normal Tissue
Figure 9 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/24713_A_9_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000121879-PIK3CA
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IHC-P
Figure 1. Recombinant Mouse Anti-PIK3CA Antibody (clone 3C1-6F9-6C4) in IHC-P.
Immunohistochemical analysis of paraffin-embedded gastric adenocarcinoma using PI3 kinase p110 α antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
IHC-P
Figure 2. Recombinant Mouse Anti-PIK3CA Antibody (clone 3C1-6F9-6C4) in IHC-P.
Immunohistochemical analysis of paraffin-embedded gastric adenocarcinoma using PI3 kinase p110 α antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- Synthetic peptide derived from human PIK3CA H1047R.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human PIK3CA
- Species Reactivity
- Human
- Clone
- 3C1-6F9-6C4
- Applications
- Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.3, 50% glycerol, 0.5% BSA
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry-Paraffin: 1/100-1/500
Target
- Alternative Names
- Phosphatidylinositol-4,5-Bisphosphate 3-Kinase Catalytic Subunit Alpha; Phosphoinositide-3-Kinase, Catalytic, Alpha Polypeptide; Serine/Threonine Protein Kinase PIK3CA; PtdIns-3-Kinase Subunit P110-Alpha; PI3K-Alpha; Phosphatidylinositol-4,5-Bisphosphate 3-Kinase Catalytic Subunit, Alpha Isoform; Phosphatidylinositol 4,5-Bisphosphate 3-Kinase Catalytic Subunit Alpha Isoform; Phosphatidylinositol-4,5-Bisphosphate 3-Kinase 110 KDa Catalytic Subunit Alpha; Phosphatidylinositol 4,5-Bisphosphate 3-Kinase 110 KDa Catalytic Subunit Alpha; Phosphatidylinositol-4,5-Bisphosphate 3-Kinase, Catalytic Subunit Alpha; Phosphatidylinositol 3-Kinase, Catalytic, Alpha Polypeptide; Phosphatidylinositol 3-Kinase, Catalytic, 110-KD, Alpha; Phosphoinositide-3-Kinase Catalytic Alpha Polypeptide; PI3-Kinase P110 Subunit Alpha; PtdIns-3-Kinase Subunit Alpha
- Gene ID
- 5290
- UniProt ID
- P42336
- Sequence Similarities
- Belongs to the PI3/PI4-kinase family.
- Cellular Localization
- Cytoplasm, Cytosol, Intercalated disc, Perinuclear region of cytoplasm, Plasma membrane
- Protein Refseq
- NP_006209.2
- Function
- Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol (PI) and its phosphorylated derivatives at position 3 of the inositol ring to produce 3-phosphoinositides (PubMed:15135396, PubMed:23936502, PubMed:28676499).
Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) (PubMed:15135396, PubMed:28676499).
PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation through the PDPK1-AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. In addition to its lipid kinase activity, it displays a serine-protein kinase activity that results in the autophosphorylation of the p85alpha regulatory subunit as well as phosphorylation of other proteins such as 4EBP1, H-Ras, the IL-3 beta c receptor and possibly others (PubMed:23936502, PubMed:28676499).
Plays a role in the positive regulation of phagocytosis and pinocytosis (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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| CAT | Product Name | Application | Type |
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| VS-0325-XY1650 | Anti-PIK3CA Immunohistochemistry Kit | IHC | |
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