Recombinant Mouse Anti-POU2F1 Antibody (clone 27-7G1)
CAT#: VS3-FY1079
This product is a recombinant mouse antibody that recognizes POU2F1. This antibody has been reported for use in Western Blot, Immunohistochemistry, Immunofluorescence. The clone 27-7G1 is specific for human POU2F1.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human POU2F1
- Species Reactivity
- Human
- Clone
- 27-7G1
- Applications
- Western Blot, Immunohistochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- 90 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- 1 mg/mL
- Buffer
- PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Immunohistochemistry-Paraffin: 1/50-300
Immunofluorescence: 1/200
Target
- Alternative Names
- NF-A1; Oct-1; OCT1; Octamer-binding protein 1; Octamer-binding transcription factor 1
- Gene ID
- 5451
- UniProt ID
- P14859
- Sequence Similarities
- Belongs to the POU transcription factor family. Class-2 subfamily.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphorylated by PRKDC.
- Protein Refseq
- NP_001185712.1; NP_001185715.1; NP_002688.3
- Function
- Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR.
(Microbial infection) In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
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- Product Name
Isotype Control
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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