Recombinant Mouse Anti-POU2F1 Antibody (clone 27-7G1) (CAT#: VS3-FY1079)

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• Human POU2F1

• Species Reactivity
• Human

• Clone
• 27-7G1

• Applications
• Western Blot, Immunohistochemistry, Immunofluorescence

• Conjugate
• Unconjugated

• MW
• 90 kDa

• Purification
• Affinity Purified

• Purity
• >95% as determined by SDS-PAGE

• Concentration
• 1 mg/mL

• Buffer
• PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

• Preservative
• 0.02% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/2000
  Immunohistochemistry-Paraffin: 1/50-300
  Immunofluorescence: 1/200

• Alternative Names
• NF-A1; Oct-1; OCT1; Octamer-binding protein 1; Octamer-binding transcription factor 1

• Gene ID
• 5451

• UniProt ID
• P14859

• Sequence Similarities
• Belongs to the POU transcription factor family. Class-2 subfamily.

• Cellular Localization
• Nucleus

• Post Translation Modifications
• Phosphorylated by PRKDC.

• Protein Refseq
• NP_001185712.1; NP_001185715.1; NP_002688.3

• Function
• Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR.
  (Microbial infection) In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.

Secondary Antibody

• 2ND-037CQ
• Chicken Anti-Mouse IgG (H&L) Antibody

• 2ND-039CQ
• Donkey Anti-Mouse IgG (H&L) Antibody

• 2ND-040CQ
• Goat Anti-Mouse IgG (H&L) Antibody

• 2ND-041CQ
• Llama Anti-Mouse IgG (H&L) Antibody

• 2ND-042CQ
• Rabbit Anti-Mouse IgG (H&L) Antibody

Isotype Control

• MRO-A1591-YJ
• Mouse IgG Isotype control, Polyclonal