Recombinant Rabbit Anti-POU2F2 Antibody (clone R02-5E7)
CAT#: VS3-FY1171
This product is a recombinant rabbit antibody that recognizes POU2F2. This antibody has been reported for use in Western Blot. The clone R02-5E7 is specific for human POU2F2. The antigen is human oct-2 synthetic peptide.
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line BJ
Immunofluorescent staining of human cell line BJ shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49056/1533_E11_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows moderate nuclear positivity in germinal center cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64404/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64404/147786_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2513/6476_A_7_3.jpg
Normal Tissue
Figure 5 Testis
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2513/6476_A_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2513/6476_A_7_8.jpg
Normal Tissue
Figure 7 Tonsil
Germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2513/6476_A_6_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced.
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000028277-POU2F2/subcellular
❮
❯
ICC
Figure 1. Recombinant Rabbit Anti-POU2F2 Antibody (clone R02-5E7) in ICC.
Immunocytochemical analysis of NUP155 (green) in HeLa using NUP155 Antibody and DAPI (blue).
❮
❯
Specifications
- Immunogen
- Human Oct-2 Synthetic Peptide.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human POU2F2
- Species Reactivity
- Human
- Clone
- R02-5E7
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- Calculated MW: 51 kDa; Observed MW: 51 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- POU2F2; OCT2; OTF2; POU domain; class 2; transcription factor 2; Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2; Octamer-binding protein 2; Oct-2; Octamer-binding transcription factor 2; OTF-2
- Gene ID
- 5452
- UniProt ID
- P09086
- Sequence Similarities
- Belongs to the POU transcription factor family. Class-2 subfamily.
- Cellular Localization
- Cytoplasm, Nucleus
- Protein Refseq
- NP_001193954.1; NP_001193955.1; NP_001234923.1N
- Function
- Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3') (PubMed:2904654, PubMed:7859290).
Regulates IL6 expression in B cells with POU2AF1 (By similarity).
Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression (PubMed:2901913, PubMed:2904654).
Modulates transcription transactivation by NR3C1, AR and PGR (PubMed:10480874).
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-FY1171. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "POU2F2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2795 | Hi-Affi™ Recombinant Rabbit Anti-POU2F2 Monoclonal Antibody (DS2795AB) | FC, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-CJ532 | Mouse Anti-POU2F2 Recombinant Antibody (clone PT0202) | IHC-P | Mouse IgG1, κ |
| VS3-CJ533 | Mouse Anti-POU2F2 Recombinant Antibody (clone ABT-OCT2) | IHC-P | Mouse IgG |
| VS3-FY1170 | Recombinant Mouse Anti-POU2F2 Antibody (clone 2B8-2E6-3A8) | IHC-P | Mouse IgG1 |
| VS3-FY1629 | Rabbit Anti-POU2F2 Recombinant Antibody (clone R07-8H6) | WB | Rabbit IgG |
| VS3-XY3474 | Rabbit Anti-POU2F2 Recombinant Antibody (clone R09-8M8) | WB, ICC, IF, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5582 | Anti-POU2F2 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, IHC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: WB, IHC, FC, Cyt, ELISA
Application: ELISA, IP, WB, IHC, IF, FuncS
Application: WB, IF, FuncS
-CB2006C17L-4.jpg)
Application: WB, ELISA
-YJ5.png)
Application: WB, ELISA, FC, IHC, IP
Application: WB, IHC, ELISA, FC
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: WB, FC, IF, Inhib, ELISA, IHC
Application: EM, ELISA, ICC, IHC-Fr, IHC-P, WB
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
