Recombinant Rabbit Anti-POU2F2 Antibody (clone R02-5E7) (CAT#: VS3-FY1171)
This product is a recombinant rabbit antibody that recognizes POU2F2. This antibody has been reported for use in Western Blot. The clone R02-5E7 is specific for human POU2F2. The antigen is human oct-2 synthetic peptide.
Specific Inquiry
ICC
Figure 1. Recombinant Rabbit Anti-POU2F2 Antibody (clone R02-5E7) in ICC.
Immunocytochemical analysis of NUP155 (green) in HeLa using NUP155 Antibody and DAPI (blue).
Specifications
- Immunogen
- Human Oct-2 Synthetic Peptide.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human POU2F2
- Species Reactivity
- Human
- Clone
- R02-5E7
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- Calculated MW: 51 kDa; Observed MW: 51 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- POU2F2; OCT2; OTF2; POU domain; class 2; transcription factor 2; Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2; Octamer-binding protein 2; Oct-2; Octamer-binding transcription factor 2; OTF-2
- Gene ID
- 5452
- UniProt ID
- P09086
- Sequence Similarities
- Belongs to the POU transcription factor family. Class-2 subfamily.
- Cellular Localization
- Cytoplasm, Nucleus
- Protein Refseq
- NP_001193954.1; NP_001193955.1; NP_001234923.1N
- Function
- Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3') (PubMed:2904654, PubMed:7859290).
Regulates IL6 expression in B cells with POU2AF1 (By similarity).
Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression (PubMed:2901913, PubMed:2904654).
Modulates transcription transactivation by NR3C1, AR and PGR (PubMed:10480874).
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2795 | Hi-Affi™ Recombinant Rabbit Anti-POU2F2 Monoclonal Antibody (DS2795AB) | FC, IHC-P, WB | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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