Recombinant Rabbit Anti-PPIB Antibody (clone R01-2F8)
CAT#: VS3-FY1174
This product is a recombinant rabbit antibody that recognizes PPIB. This antibody has been reported for use in Western Blot. The clone R01-2F8 is specific for human, mouse, rat PPIB. The antigen is a synthetic peptide of human cyclophilin b.
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line HUVEC TERT2
Immunofluorescent staining of human cell line HUVEC TERT2 shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12720/1752_C1_2_cr5804bfa7d57a4_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11487/30837_B_9_5.jpg
Normal Tissue
Figure 3 Thyroid gland
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11487/30837_B_1_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Enterocytes
Staining: Low
Intensity: Weak
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11487/30837_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11487/30837_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11487/30837_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11487/30837_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11487/30837_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000166794-PPIB/subcellular
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WB
Figure 1. Recombinant Rabbit Anti-PPIB Antibody (clone R01-2F8) in WB.
Western Blot analysis of cyclophilin B in HeLa, C6, rat brain lysates using cyclophilin B antibody.
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❯
Specifications
- Immunogen
- A synthetic peptide of human cyclophilin B.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat PPIB
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-2F8
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- Calculated MW: 24 kDa; Observed MW: 20 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- PPIB; CYPB; Peptidyl-prolyl cis-trans isomerase B; PPIase B; CYP-S1; Cyclophilin B; Rotamase B; S-cyclophilin; SCYLP
- Gene ID
- 5479
- UniProt ID
- P23284
- Sequence Similarities
- Belongs to the cyclophilin-type PPIase family. PPIase B subfamily.
- Cellular Localization
- Endoplasmic reticulum, Virion
- Protein Refseq
- NP_000933.1
- Function
- PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "PPIB"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2805 | Hi-Affi™ Recombinant Rabbit Anti-PPIB Monoclonal Antibody (DS2805AB) | WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0420-CN | Recombinant Rabbit Anti-PPIB Monoclonal Antibody (CBACN-169) | WB | Rabbit IgG |
| ZG-0207J | Mouse Anti-PPIB Recombinant Antibody (clone 1B3) | WB, IHC-P, IF | Mouse IgG |
| ZG-0208J | Mouse Anti-PPIB Recombinant Antibody (clone 2B10) | IF, ICC, WB, IHC-P | Mouse IgG |
| VS3-CJ793 | Mouse Anti-PPIB Recombinant Antibody (VS3-CJ793) | WB, IHC | Mouse IgG |
| VS3-CJ794 | Rabbit Anti-PPIB Recombinant Antibody (VS3-CJ794) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1427 | AbPlus™ Anti-PPIB Magnetic Beads (VS-0724-YC1427) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1709 | Anti-PPIB Immunohistochemistry Kit | IHC | |
| VS-0525-XY5596 | Anti-Human PPIB Immunohistochemistry Kit | IHC |
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