Recombinant Mouse Anti-PPP4C Antibody (VS4-CJ86) (CAT#: VS4-CJ86)

• Immunogen
• Recombinant full length protein (307aa) of human PPP4C.

• Host Species
• Mouse

• Type
• Mouse IgG2a

• Specificity
• Human, Dog, Mouse, Bovine, Rat PPP4C

• Species Reactivity
• Human, Dog, Mouse, Bovine, Rat

• Applications
• ELISA, WB, IHC, ICC

• Conjugate
• Unconjugated

• Purification
• Affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 50% Glycerol, 0.1M Tris-Glycine (150 mM NaCl), pH 7.4.

• Preservative
• 0.2% Sodium Azide

• Storage
• Store at -20°C or -80°C upon receipt. Avoid repeated freeze.

• Application Notes
• This antibody has been tested for use in ELISA, Western Blot (1:500-1:5000), Immunohistochemistry (1:50-1:500), Immunocytochemistry (1:50-1:200).

• Alternative Names
• PP-X; PP4; PP4C; PPH3; PPP4; PPX

• Gene ID
• 5531

• UniProt ID
• P60510

• Long Name
• Protein phosphatase 4 catalytic subunit

• Sequence Similarities
• Belongs to the PPP phosphatase family. PP-4 (PP-X) subfamily.

• Cellular Localization
• Nucleus, Cytoplasm, Cytoskeleton

• Post Translation Modifications
• Methylation at the C-terminal Leu-307 is critical for interactions with regulatory subunits and functions in DNA repair.

• Protein Refseq
• NP_001290432.1; NP_001290433.1; NP_001290435.1

• Function
• Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AX phosphorylated on Ser-140 (gamma-H2AX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity).
  In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin.

Secondary Antibody

• 2RD-A1464-YJ
• Goat Anti-Mouse IgG2a Secondary antibody

• 2RD-A1564-YJ
• Rabbit Anti-Mouse IgG2a Secondary antibody (Clone RM219)