Recombinant Rabbit Anti-PRKCG (phospho Thr514) Antibody (clone R02-4F3) (CAT#: VS3-FY1194)
This product is a recombinant rabbit antibody that recognizes PRKCG. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation. The clone R02-4F3 is specific for human, mouse, rat PRKCG. The antigen is a synthetic phosphopeptide corresponding to residues surrounding thr514 of human pkc.
Specific Inquiry
WB
Figure 1. Recombinant Rabbit Anti-PRKCG (phospho Thr514) Antibody (clone R02-4F3) in WB.
Western Blot analysis of Phospho-PKC gamma (Thr514) in C6, Hela lysates using Phospho-PKC gamma (Thr514) Antibody.
IHC-P
Figure 2. Recombinant Rabbit Anti-PRKCG (phospho Thr514) Antibody (clone R02-4F3) in IHC-P.
Immunohistochemical analysis of paraffin-embedded human brain using PKC (Phospho-Thr514) antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
Specifications
- Immunogen
- A synthetic phosphopeptide corresponding to residues surrounding Thr514 of human PKC.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat PRKCG
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R02-4F3
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 78 kDa; Observed MW: 78-85 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunoprecipitation: 1/20
Target
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3038z | Mouse Anti-PRKCG Recombinant Antibody (clone 5G8) | WB, ELISA, ICC, IP | Mouse IgG1 |
| ZG-0415U | Rabbit Anti-PRKCG Recombinant Antibody (clone K21-L), FITC | FC | Rabbit IgG |
| VS3-FY1195 | Recombinant Rabbit Anti-PRKCG (phospho Thr410) Antibody (clone R03-3D0) | WB, IHC-P | Rabbit IgG |
| VS3-FY1835 | Rabbit Anti-PRKCG Recombinant Antibody (clone R06-8C6) | WB, IP | Rabbit IgG |
| VS3-WK2263 | Rabbit Anti-PRKCG (pT514) Recombinant Antibody (VS3-WK2263) | WB, IHC, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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