Recombinant Rabbit Anti-PRKCG (phospho Thr514) Antibody (clone R02-4F3) (CAT#: VS3-FY1194)
This product is a recombinant rabbit antibody that recognizes PRKCG. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation. The clone R02-4F3 is specific for human, mouse, rat PRKCG. The antigen is a synthetic phosphopeptide corresponding to residues surrounding thr514 of human pkc.
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WB
Figure 1. Recombinant Rabbit Anti-PRKCG (phospho Thr514) Antibody (clone R02-4F3) in WB.
Western Blot analysis of Phospho-PKC gamma (Thr514) in C6, Hela lysates using Phospho-PKC gamma (Thr514) Antibody.
IHC-P
Figure 2. Recombinant Rabbit Anti-PRKCG (phospho Thr514) Antibody (clone R02-4F3) in IHC-P.
Immunohistochemical analysis of paraffin-embedded human brain using PKC (Phospho-Thr514) antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
Specifications
- Immunogen
- A synthetic phosphopeptide corresponding to residues surrounding Thr514 of human PKC.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat PRKCG
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R02-4F3
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 78 kDa; Observed MW: 78-85 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunoprecipitation: 1/20
Target
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3038z | Mouse Anti-PRKCG Recombinant Antibody (clone 5G8) | WB, ELISA, ICC, IP | Mouse IgG1 |
| MRO-1240-CN | Recombinant Rabbit Anti-PRKCG Monoclonal Antibody (JG69-32) | WB, IHC | Rabbit IgG |
| ZG-0415U | Rabbit Anti-PRKCG Recombinant Antibody (clone K21-L), FITC | FC | Rabbit IgG |
| VS3-FY1835 | Rabbit Anti-PRKCG Recombinant Antibody (clone R06-8C6) | WB, IP | Rabbit IgG |
| VS3-WK2263 | Rabbit Anti-PRKCG (pT514) Recombinant Antibody (VS3-WK2263) | WB, IHC, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
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