Recombinant Rabbit Anti-PTBP1 Antibody (clone R01-1G2)
CAT#: VS3-FY1210
This product is a recombinant rabbit antibody that recognizes PTBP1. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin. The clone R01-1G2 is specific for human, mouse, Hamster PTBP1. The antigen is synthetic peptide of human ptbp1.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13507/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13507/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13507/30256_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13507/30256_A_7_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13507/30256_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Peritubular cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13507/30256_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13507/30256_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000011304-PTBP1/subcellular
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Specifications
- Immunogen
- Synthetic peptide of human PTBP1.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Hamster PTBP1
- Species Reactivity
- Human, Mouse, Hamster
- Clone
- R01-1G2
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
- MW
- Calculated MW: 57 kDa; Observed MW: 57 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "PTBP1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1658CT | Recombinant Mouse anti-Human PTBP1 Monoclonal antibody (EML2203) | IHC-P, WB | |
| MRO-1290-CN | Recombinant Mouse Anti-PTBP1 Monoclonal Antibody (9A1) | WB, IHC, IF, FC | Mouse IgG2a |
| MRO-1291-CN | Recombinant Mouse Anti-PTBP1 Monoclonal Antibody (5C1) | WB, IHC | Mouse IgG1 |
| MRO-1292-CN | Recombinant Rabbit Anti-PTBP1 Monoclonal Antibody (JU41-20) | WB, IHC | Rabbit IgG |
| ZG-0526U | Rabbit Anti-PTBP1 Recombinant Antibody (clone 8A1) | ELISA, WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2911 | Hi-Affi™ Recombinant Rabbit Anti-PTBP1 Monoclonal Antibody (DS2911AB) | WB, IHC, ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC2086 | PE-B*07:02/Human PTBP1 (SPNQARAQAAL) MHC Tetramer | FCM | |
| MHC-LC2087 | APC-B*07:02/Human PTBP1 (SPNQARAQAAL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC963 | AbPlus™ Anti-PTBP1 Magnetic Beads (VS-0724-YC963) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1815 | Anti-PTBP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5812 | Anti-Mouse PTBP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5811 | Anti-Human PTBP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5813 | Anti-Rat PTBP1 Immunohistochemistry Kit | IHC |
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