Recombinant Rabbit Anti-PTK2 Antibody (clone R08-3C1)
CAT#: VS3-FY1221
This product is a recombinant rabbit antibody that recognizes PTK2. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin. The clone R08-3C1 is specific for human PTK2. The antigen is human fak synthetic peptide.
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & focal adhesion sites.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human urinary bladder
Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic and membranous positivity in urothelial cells
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_A_6_5_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_A_6_6.jpg
Normal Tissue
Figure 8 Breast
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Myoepithelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_B_1_4.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_A_9_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000169398-PTK2/subcellular
❮
❯
WB
Figure 1. Recombinant Rabbit Anti-PTK2 Antibody (clone R08-3C1) in WB.
Western Blot analysis of FAK in MCF-7, T47D, HCT116 lysates using FAK antibody.
❮
❯
Specifications
- Immunogen
- Human FAK Synthetic Peptide.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PTK2
- Species Reactivity
- Human
- Clone
- R08-3C1
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
- MW
- Calculated MW: 119 kDa; Observed MW: 125 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-FY1221. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "PTK2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2031z | Mouse Anti-PTK2 Recombinant Antibody (clone 11B8) | WB, ELISA, ICC, IHC | Mouse IgG1 |
| ZG-0100U | Rabbit Anti-PTK2 Recombinant Antibody (clone 3B2) | ELISA, IHC | Rabbit IgG |
| ZG-0531U | Rabbit Anti-Phospho-PTK2 (Y397) Recombinant Antibody (clone 1B3) | ELISA, WB | Rabbit IgG |
| VS3-XY1315 | Mouse Anti-PTK2 Recombinant Antibody (clone 4G1) | WB, IP, IF, IHC | Mouse IgG1 |
| VS3-WK2271 | Rabbit Anti-PTK2 (pY576) Recombinant Antibody (VS3-WK2271) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0748MZ | Chicken Anti-FAK1 Polyclonal IgY | ELISA, IHC, IP, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2924 | Hi-Affi™ Recombinant Rabbit Anti-PTK2 Monoclonal Antibody (DS2924AB) | ICC, WB | IgG |
| MOR-4573 | Hi-Affi™ Recombinant Rabbit Anti-PTK2 Monoclonal Antibody (TH85DS) | WB, IF, ICC, FC | IgG |
| MOR-4678 | Hi-Affi™ Recombinant Rabbit Anti-PTK2 Monoclonal Antibody (TH192DS) | WB, IF, ICC, IHC-P, FC, ELISA | IgG |
| MOR-4679 | Hi-Affi™ Recombinant Rabbit Anti-PTK2 Monoclonal Antibody (TH193DS) | IF, ICC, IHC-P, FC | IgG |
| MOR-4680 | Hi-Affi™ Recombinant Rabbit Anti-PTK2 Monoclonal Antibody (TH194DS) | WB, IF, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC2719 | PE-A*02:01/Human PTK2 (SLDLASLIL) MHC Tetramer | FCM | |
| MHC-LC2720 | PE-A*02:07/Human PTK2 (SLDLASLIL) MHC Tetramer | FCM | |
| MHC-LC4514 | PE-Mamu-A1*001:01/Human PTK2 (LTAAHALAV) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY219 | AbPlus™ Anti-PTK2 Magnetic Beads (CBACN-222) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC956 | CytoStream™ Rabbit Anti-PTK2 Recombinant Antibody (VS13-YC956) | WB, ICC, IF, IHC-P, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5845 | Anti-PTK2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5846 | Anti-Mouse PTK2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5847 | Anti-Rat PTK2 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: FC, IB, Block, Inhib, FuncS, ELISA, FACS, IP, IF
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: ELISA, IHC, FC, IP, IF, FuncS
-3.jpg)
Application: Neut
Application: ELISA, FC, Inhib, IHC-Fr, WB, IP
Application: ELISA, Inhib, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
