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Recombinant Rabbit Anti-RAD51 Antibody (clone R04-3C6)

Recombinant Rabbit Anti-RAD51 Antibody (clone R04-3C6) (CAT#: VS3-FY1243)

Name: Recombinant Rabbit Anti-RAD51 Antibody (clone R04-3C6)
SKU: VS3-FY1243
Rating: 5 (1 reviews)

This product is a recombinant rabbit antibody that recognizes RAD51. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence. The clone R04-3C6 is specific for human RAD51. The antigen is human rad51 synthetic peptide.

Tested Data
Gene Expression
Datasheet
MSDS
COA

ICC

Figure 1. Recombinant Rabbit Anti-RAD51 Antibody (clone R04-3C6) in ICC.

Figure 1. Recombinant Rabbit Anti-RAD51 Antibody (clone R04-3C6) in ICC.

Immunocytochemical analysis of Rad51 (green) in Jurkat using Rad51 Antibody and DAPI (blue).

Subcellular Location

Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli rim & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39310/493_D2_3_selected.jpg

Normal Tissue

Figure 2 Testis
(Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Leydig cells Staining: Medium Intensity: Strong Quantity: <25% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: Low Intensity: Weak Quantity: 75%-25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Medium Intensity: Strong Quantity: <25% Spermatogonia cells Staining: Low Intensity: Weak)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39310/91890_A_6_6.jpg

Figure 3 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39310/91890_B_8_5.jpg

Figure 4 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39310/91890_A_7_3.jpg

RNA Expression

Figure 5 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000051180-RAD51

Datasheet

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Human Rad51 synthetic peptide.

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human RAD51

Species Reactivity
Human

Clone
R04-3C6

Applications
Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence

Conjugate
Unconjugated

MW
Calculated MW: 37 kDa; Observed MW: 37 kDa

Product Property

Purification
Affinity Purified

Purity
>95% as determined by SDS-PAGE

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200

Target

Alternative Names
RAD51 Recombinase; BRCA1/BRCA2-Containing Complex, Subunit 5; RAD51 Homolog A; HsRad51; HRAD51; RAD51A; RECA; RAD51 Homolog (RecA Homolog, E. Coli) (S. Cerevisiae); RAD51 (S. Cerevisiae) Homolog (E Coli RecA Homolog); DNA Repair Protein RAD51 Homolog 1

Gene ID
5888

UniProt ID
Q06609

Secondary Antibody

Isotype Control

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "RAD51"

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IgG Antibody Products Rabbit Monoclonal Antibody Products Antibody Magnetic Beads

CAT	Product Name	Application	Type
MOB-3172z	Mouse Anti-RAD51 Recombinant Antibody (clone 12G11)	WB, ICC, IF, IHC, IP	Mouse IgG2b
MRO-1314-CN	Recombinant Rabbit Anti-RAD51 Monoclonal Antibody (JM54-26)	WB, IF, IHC, IP, FC	Rabbit IgG
ZG-051R	Mouse Anti-Rad51 Recombinant Antibody (ZG-051R)	WB	Mouse IgG
VS3-WK595	Rabbit Anti-RAD51 Recombinant Antibody (clone JM54-26)	WB, ELISA	Rabbit IgG
VS3-FY1242	Recombinant Rabbit Anti-RAD51 Antibody (clone R06-5G2)	WB, ICC, IF, IP	Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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