Recombinant Rabbit Anti-RBM3 Antibody (clone R02-0C1)
CAT#: VS3-FY1257
This product is a recombinant rabbit antibody that recognizes RBM3. This antibody has been reported for use in Western Blot, Immunocytochemistry, Immunofluorescence. The clone R02-0C1 is specific for human, mouse RBM3. The antigen is synthetic peptide of human rbm3.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3624/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/62559/1538_B6_4_selected.jpg
Normal Tissue
Figure 3 IHC staining of human skin
Immunohistochemical staining of human skin shows moderate nuclear positivity in epidermal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3624/ihc_selected.jpg
Normal Tissue
Figure 4 Tonsil.
High expression in tonsil
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/62559/144678_A_6_8_val_selected.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Pachytene spermatocytes Staining: Low Intensity: Weak Quantity: 75%-25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/62559/144678_A_6_6.jpg
Normal Tissue
Figure 6 Esophagus
Squamous epithelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/62559/144678_B_5_1.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000102317-RBM3
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Specifications
- Immunogen
- Synthetic peptide of human RBM3.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse RBM3
- Species Reactivity
- Human, Mouse
- Clone
- R02-0C1
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 17 kDa; Observed MW: 17 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "RBM3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4017z | Mouse Anti-RBM3 Recombinant Antibody (clone 18G3) | WB, ELISA, IHC | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3022 | Hi-Affi™ Recombinant Rabbit Anti-RBM3 Monoclonal Antibody (DS3022AB) | WB, ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC992 | CytoStream™ Rabbit Anti-RBM3 Recombinant Antibody (VS13-YC992) | ICC, IF, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6032 | Anti-RBM3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6033 | Anti-Mouse RBM3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6034 | Anti-Rat RBM3 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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