Recombinant Rabbit Anti-RNF2 Antibody (clone R04-2B3)
CAT#: VS3-FY1274
This product is a recombinant rabbit antibody that recognizes RNF2. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin. The clone R04-2B3 is specific for human RNF2. The antigen is synthetic peptides derived from human ring2 / ring1b / rnf2.
Gene Expression
Tested Data
Normal Tissue
Figure 1 IHC staining of human urinary bladder
Immunohistochemical staining of human urinary bladder shows nuclear positivity in urothelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26803/62883_A_5_5_selected.jpg
Normal Tissue
Figure 1 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glandular cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26803/62883_A_9_3.jpg
Normal Tissue
Figure 2 Liver
Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26803/62883_A_9_4.jpg
Normal Tissue
Figure 3 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26803/62883_A_8_5.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26803/62883_A_6_6.jpg
RNA Expression
Figure 5 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000121481-RNF2
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IHC-P
Figure 1. Recombinant Rabbit Anti-RNF2 Antibody (clone R04-2B3) in IHC-P.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using RING2/RING1B/RNF2 Antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- Synthetic peptides derived from human RING2 / RING1B / RNF2.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human RNF2
- Species Reactivity
- Human
- Clone
- R04-2B3
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
- MW
- Calculated MW: 38 kDa; Observed MW: 38 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "RNF2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3133z | Mouse Anti-RNF2 Recombinant Antibody (clone 8G8) | WB, ELISA, ICC, IF | Mouse IgG2a, κ |
| MRO-1339-CN | Recombinant Rabbit Anti-RNF2 Monoclonal Antibody (JB38-41) | WB, IHC | Rabbit IgG |
| VS3-WK293 | Rabbit Anti-RNF2 Recombinant Antibody (clone JB38-41) | WB, ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3084 | Hi-Affi™ Recombinant Rabbit Anti-RNF2 Monoclonal Antibody (DS3084AB) | WB, ICC, IF | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC388 | AbPlus™ Anti-RNF2 Magnetic Beads (VS-0724-YC388) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6160 | Anti-RNF2 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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