Recombinant Rabbit Anti-RPS6KA1 (phospho Thr359/Ser363) Antibody (clone R01-5B1)
CAT#: VS3-FY1281
This product is a recombinant rabbit antibody that recognizes RPS6KA1. This antibody has been reported for use in Western Blot, Immunoprecipitation. The clone R01-5B1 is specific for human, mouse, rat RPS6KA1. The antigen is a synthetic phosphopeptide corresponding to residues surrounding thr359/ser363 of human rsk1 p90.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7981/21_H4_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7981/23318_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7981/23318_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7981/23318_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7981/23318_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Preleptotene spermatocytes
Staining: Low
Intensity: Moderate
Quantity: <25%
Round or early spermatids
Staining: Low
Intensity: Moderate
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7981/23318_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7981/23318_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (HMC-1, U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000117676-RPS6KA1/subcellular
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Specifications
- Immunogen
- A synthetic phosphopeptide corresponding to residues surrounding Thr359/Ser363 of human RSK1 p90.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat RPS6KA1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-5B1
- Applications
- Western Blot, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 83 kDa; Observed MW: 90 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "RPS6KA1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0827MZ | Recombinant Mouse Anti-Human RPS6KA1 Antibody (clone 8C12) | ELISA | Mouse antibody |
| MRO-2372-CN | Recombinant Rabbit Anti-RPS6KA1 (phosphorylated Ser380) Monoclonal Antibody (CBACN-658) | WB, IF, IP | Rabbit IgG |
| MRO-2373-CN | Recombinant Rabbit Anti-RPS6KA1 (phosphorylated Thr359+S363) Monoclonal Antibody (CBACN-659) | WB, IF, IHC, IP | Rabbit IgG |
| ZG-0552U | Rabbit Anti-Phospho-RPS6KA1 (T359+S363) Recombinant Antibody (clone 1B6) | ELISA, WB, IF, IP | Rabbit IgG |
| ZG-0553U | Rabbit Anti-Phospho-RPS6KA1 (S380) Recombinant Antibody (clone 1E11) | ELISA, WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3121 | Hi-Affi™ Recombinant Rabbit Anti-RPS6KA1 Monoclonal Antibody (DS3121AB) | ICC, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY227 | AbPlus™ Anti-RPS6KA1 Magnetic Beads (CBACN-658) | IP, Protein Purification | |
| VS-0724-YC1347 | AbPlus™ Anti-RPS6KA1 Magnetic Beads (VS-0724-YC1347) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1018 | CytoStream™ Rabbit Anti-RPS6KA1 Recombinant Antibody (VS13-YC1018) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6219 | Anti-RPS6KA1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6220 | Anti-Mouse RPS6KA1 Immunohistochemistry Kit | IHC |
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