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  • Recombinant Rabbit Anti-RPS6KA3 (phospho Ser227) Antibody (clone R01-5B5)

Recombinant Rabbit Anti-RPS6KA3 (phospho Ser227) Antibody (clone R01-5B5) (CAT#: VS3-FY1282)

This product is a recombinant rabbit antibody that recognizes RPS6KA3. This antibody has been reported for use in Western Blot, Immunoprecipitation. The clone R01-5B5 is specific for human, mouse, rat RPS6KA3. The antigen is a synthetic phosphopeptide corresponding to residues surrounding ser227 of human rsk 2/mapkap kinase 1b.

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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, nucleoli & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3221/91_C11_2_selected.jpg
Normal Tissue
Figure 2 Lymph node
Figure 2 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3221/9882_A_8_8.jpg
Figure 3 Cerebral cortex
Figure 3 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3221/9882_B_8_5.jpg
Figure 4 Colon
Figure 4 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3221/9882_A_8_3.jpg
Figure 5 Liver
Figure 5 Liver
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3221/9882_A_9_4.jpg
Figure 6 Kidney
Figure 6 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3221/9882_A_8_5.jpg
Figure 7 Testis
Figure 7 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3221/9882_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (Hep G2)
Figure 8 RNA cell line category: Cell line enhanced (Hep G2)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000177189-RPS6KA3

Specifications

  • Immunogen
  • A synthetic phosphopeptide corresponding to residues surrounding Ser227 of human Rsk 2/MAPKAP kinase 1b.
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse, Rat RPS6KA3
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R01-5B5
  • Applications
  • Western Blot, Immunoprecipitation
  • Conjugate
  • Unconjugated
  • MW
  • Calculated MW: 84 kDa; Observed MW: 84 kDa

Product Property

  • Purification
  • Affinity Purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Buffer
  • 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
  • Preservative
  • 0.01% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunoprecipitation: 1/20

Target

  • Alternative Names
  • Ribosomal Protein S6 Kinase A3; MAP Kinase-Activated Protein Kinase 1b; Insulin-Stimulated Protein Kinase 1; MAPK-Activated Protein Kinase 1b; Ribosomal S6 Kinase 2; MAPKAP Kinase 1b; S6K-Alpha-3; EC 2.7.11.1; MAPKAPK-1b; P90-RSK 3; MAPKAPK1B; Pp90RSK2; ISPK-1; RSK-2; RSK2

Recommended Products

Secondary Antibody

Isotype Control

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "RPS6KA3"

Select a product category from the dropdown menu below to view related products.
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IgG Antibody Products Rabbit Monoclonal Antibody Products
CAT Product Name Application Type
MOB-3927z Mouse Anti-RPS6KA3 Recombinant Antibody (clone 18G7) WB, ELISA, FC, IF Mouse IgG1
VS3-QX984 Mouse Anti-RPS6KA3 Recombinant Antibody (clone 5H5-C11-F10) WB, IHC, ICC, FC, ELISA Mouse IgG1
VS3-XY1386 Mouse Anti-RPS6KA3 Recombinant Antibody (clone 356CT10.6.1.2) WB Mouse IgG1
VS3-FY1283 Recombinant Rabbit Anti-RPS6KA3 Antibody (clone R05-6E3) WB, IHC-P, IP Rabbit IgG
VS3-WK2265 Rabbit Anti-RPS6KA3 (pS227) Recombinant Antibody (VS3-WK2265) WB, IP Rabbit IgG

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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