Recombinant Rabbit Anti-RPS6KB2 (phospho Ser371) Antibody (clone R01-4A5) (CAT#: VS3-FY1286)
This product is a recombinant rabbit antibody that recognizes RPS6KB2. This antibody has been reported for use in Western Blot. The clone R01-4A5 is specific for human, mouse, rat RPS6KB2. The antigen is a synthetic phosphopeptide corresponding to residues surrounding ser371 of human p70 s6 kinase β.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19404/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19404/if_selected.jpg
Figure 2 Cerebral cortex
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10010/24830_B_7_5.jpg
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10010/24830_B_7_5.jpg
Figure 3 Colon
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10010/24830_A_7_3.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10010/24830_A_7_3.jpg
Figure 4 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10010/24830_A_8_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10010/24830_A_8_5.jpg
Figure 5 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000175634-RPS6KB2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000175634-RPS6KB2
Specifications
- Immunogen
- A synthetic phosphopeptide corresponding to residues surrounding Ser371 of human P70 S6 kinase β.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat RPS6KB2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-4A5
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- Calculated MW: 53 kDa; Observed MW: 60 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Ribosomal Protein S6 Kinase B2; 70 KDa Ribosomal Protein S6 Kinase 2; Serine/Threonine-Protein Kinase 14B; P70 Ribosomal S6 Kinase Beta; S6 Kinase-Related Kinase; P70 S6 Kinase Beta; P70 S6K-Beta; EC 2.7.11.1; S6K-Beta-2; P70-S6K 2; P70 S6KB; P70-Beta; S6K-Beta; P70S6K2; STK14B
- Gene ID
- 6199
- UniProt ID
- Q9UBS0
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "RPS6KB2"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1518CT | Recombinant Mouse anti-Human RPS6KB2 Monoclonal antibody (4B11) | ELISA, WB | |
| MRO-2105-CN | Rabbit Anti-RPS6KB2 Polyclonal Antibody (MRO-2105-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-QX987 | Mouse Anti-RPS6KB2 Recombinant Antibody (clone 164CT21.2.2) | WB | Mouse IgG1, κ |
| VS3-XY1388 | Mouse Anti-RPS6KB2 Recombinant Antibody (clone 2G1) | WB, IP, IF, IHC | Mouse IgG1 |
| VS3-FY1287 | Recombinant Rabbit Anti-RPS6KB2 Antibody (clone R04-9C1) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3127 | Hi-Affi™ Recombinant Rabbit Anti-RPS6KB2 Monoclonal Antibody (DS3127AB) | WB | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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