Recombinant Mouse Anti-S100A1 Antibody (clone 9E8-8B8-9B4)
CAT#: VS3-FY1292
This product is a recombinant mouse antibody that recognizes S100A1. This antibody has been reported for use in Immunohistochemistry-Paraffin. The clone 9E8-8B8-9B4 is specific for human S100A1. The antigen is synthetic peptide derived from human s100.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm, cytosol & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6462/1662_G4_2_selected.jpg
Normal Tissue
Figure 2 Heart muscle
Cardiomyocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6462/20242_B_4_6.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6462/20242_B_9_5.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6462/20242_A_9_5.jpg
RNA Expression
Figure 5 RNA cell line category: Group enriched (Hep G2, SK-MEL-30)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000160678-S100A1
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IHC-P
Figure 1. Recombinant Mouse Anti-S100A1 Antibody (clone 9E8-8B8-9B4) in IHC-P.
Immunohistochemical analysis of paraffin-embedded malignant melanoma using S100A1 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- Synthetic peptide derived from human S100.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human S100A1
- Species Reactivity
- Human
- Clone
- 9E8-8B8-9B4
- Applications
- Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.3, 50% glycerol, 0.5% BSA
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry-Paraffin: 1/100-1/500
Target
- Alternative Names
- S100 Calcium Binding Protein A1; S100 Calcium-Binding Protein A1; S-100 Protein Subunit Alpha; S-100 Protein Alpha Chain; S100A; S100 Protein, Alpha Polypeptide
- Gene ID
- 6271
- UniProt ID
- P23297
- Sequence Similarities
- Belongs to the S-100 family.
- Cellular Localization
- Cytoplasm, Mitochondrion, Sarcoplasmic reticulum
- Post Translation Modifications
- Glutathionylated; glutathionylation increases affinity to calcium about 10-fold.
- Protein Refseq
- NP_006262.1
- Function
- Small calcium binding protein that plays important roles in several biological processes such as Ca2+ homeostasis, chondrocyte biology and cardiomyocyte regulation (PubMed:12804600).
In response to an increase in intracellular Ca2+ levels, binds calcium which triggers conformational changes (PubMed:23351007).
These changes allow interactions with specific target proteins and modulate their activity (PubMed:22399290).
Regulates a network in cardiomyocytes controlling sarcoplasmic reticulum Ca2+ cycling and mitochondrial function through interaction with the ryanodine receptors RYR1 and RYR2, sarcoplasmic reticulum Ca2+-ATPase/ATP2A2 and mitochondrial F1-ATPase (PubMed:12804600).
Facilitates diastolic Ca2+ dissociation and myofilament mechanics in order to improve relaxation during diastole (PubMed:11717446).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
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- Product Name
Isotype Control
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Reconstitution Buffer
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Recommended Dilution Buffer
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1444MZ | Recombinant Mouse Anti-Human S100A1 Antibody (clone 9C20) | ELISA, ICC, IF, WB, sELISA | Mouse antibody |
| ZG-0390U | Rabbit Anti-S100A1 Recombinant Antibody (clone Q17-N) | ICC | Rabbit IgG |
| VS3-CJ795 | Mouse Anti-S100A1 Recombinant Antibody (clone PT0234) | IHC-P | Mouse IgG1, κ |
| VS3-CJ796 | Mouse Anti-S100A1 Recombinant Antibody (clone PT1427) | IHC-P | Mouse IgG1, κ |
| VS3-CJ797 | Mouse Anti-S100A1 Recombinant Antibody (clone ABT-S100A1) | IF, IHC-P | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1986 | Anti-S100A1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6293 | Anti-Human S100A1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6294 | Anti-Mouse S100A1 Immunohistochemistry Kit | IHC |
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