Recombinant Rabbit Anti-S100A1 Capture Antibody (clone R39-3P-7)
CAT#: VS3-WK1918
This antibody is a rabbit recombinant antibody against human S100A1. It was developed by immunization of rabbit with recombinant human S100A1 protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. The sandwich ELISA was established using matched antibody pair R39-3P-7 and R68-6V-1. The clone R39-3P-7 can be used as a capture antibody in ELISA analysis to identify human S100A1.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm, cytosol & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6462/1662_G4_2_selected.jpg
Normal Tissue
Figure 2 Heart muscle
Cardiomyocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6462/20242_B_4_6.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6462/20242_B_9_5.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6462/20242_A_9_5.jpg
RNA Expression
Figure 5 RNA cell line category: Group enriched (Hep G2, SK-MEL-30)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000160678-S100A1
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Specifications
- Immunogen
- Recombinant human S100A1 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human S100A1
- Species Reactivity
- Human
- Clone
- R39-3P-7
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- S100 Calcium Binding Protein A1; S100 Calcium-Binding Protein A1; S-100 Protein Subunit Alpha; S-100 Protein Alpha Chain; S100A; S100 Protein, Alpha Polypeptide
- Gene ID
- 6271
- UniProt ID
- P23297
- Long Name
- S100 Calcium Binding Protein A1
- Sequence Similarities
- Belongs to the S-100 family.
- Cellular Localization
- Cytoplasm, Mitochondrion, Sarcoplasmic reticulum
- Post Translation Modifications
- Glutathionylated; glutathionylation increases affinity to calcium about 10-fold.
- Protein Refseq
- NP_006262.1
- Function
- Small calcium binding protein that plays important roles in several biological processes such as Ca2+ homeostasis, chondrocyte biology and cardiomyocyte regulation.
In response to an increase in intracellular Ca2+ levels, binds calcium which triggers conformational changes.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Detection antibody
Isotype Control
- CAT
- Product Name
Secondary Antibody
See other products for "S100A1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0356U | Rabbit Anti-S100A1 Recombinant Antibody (clone D28-E) | IHC-P | Rabbit IgG |
| ZG-0390U | Rabbit Anti-S100A1 Recombinant Antibody (clone Q17-N) | ICC | Rabbit IgG |
| VS3-CJ795 | Mouse Anti-S100A1 Recombinant Antibody (clone PT0234) | IHC-P | Mouse IgG1, κ |
| VS3-CJ796 | Mouse Anti-S100A1 Recombinant Antibody (clone PT1427) | IHC-P | Mouse IgG1, κ |
| VS3-CJ797 | Mouse Anti-S100A1 Recombinant Antibody (clone ABT-S100A1) | IF, IHC-P | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1986 | Anti-S100A1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6293 | Anti-Human S100A1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6294 | Anti-Mouse S100A1 Immunohistochemistry Kit | IHC |
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