Recombinant Rabbit Anti-S100A1 Detection Antibody (clone R68-6V-1) (CAT#: VS3-WK1919)
This antibody is a rabbit recombinant antibody against human S100A1. It was developed by immunization of rabbit with recombinant human S100A1 protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. R39-3P-7 and R68-6V-1 were identified as antibody pair for sandwich ELISA. As a detection antibody, the clone R68-6V-1 can be used in ELISA analysis to identify human S100A1.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line Hep G2
(Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm, cytosol & the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6462/1662_G4_2_selected.jpg
(Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm, cytosol & the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6462/1662_G4_2_selected.jpg
Figure 2 Heart muscle
(Cardiomyocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6462/20242_B_4_6.jpg
(Cardiomyocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6462/20242_B_4_6.jpg
Figure 3 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6462/20242_B_9_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6462/20242_B_9_5.jpg
Figure 4 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6462/20242_A_9_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6462/20242_A_9_5.jpg
Figure 5 RNA cell line category: Group enriched (Hep G2, SK-MEL-30)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000160678-S100A1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000160678-S100A1
Specifications
- Immunogen
- Recombinant human S100A1 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human S100A1
- Species Reactivity
- Human
- Clone
- R68-6V-1
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- S100 Calcium Binding Protein A1; S100 Calcium-Binding Protein A1; S-100 Protein Subunit Alpha; S-100 Protein Alpha Chain; S100A; S100 Protein, Alpha Polypeptide
- Gene ID
- 6271
- UniProt ID
- P23297
- Long Name
- S100 Calcium Binding Protein A1
- Sequence Similarities
- Belongs to the S-100 family.
- Cellular Localization
- Cytoplasm, Mitochondrion, Sarcoplasmic reticulum
- Post Translation Modifications
- Glutathionylated; glutathionylation increases affinity to calcium about 10-fold.
- Protein Refseq
- NP_006262.1
- Function
- Small calcium binding protein that plays important roles in several biological processes such as Ca2+ homeostasis, chondrocyte biology and cardiomyocyte regulation.
In response to an increase in intracellular Ca2+ levels, binds calcium which triggers conformational changes.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3941z | Mouse Anti-S100A1 Recombinant Antibody (clone 9H7) | WB, ELISA, IHC | Mouse IgG1 |
| VS3-CJ795 | Mouse Anti-S100A1 Recombinant Antibody (clone PT0234) | IHC-P | Mouse IgG1, κ |
| VS3-CJ796 | Mouse Anti-S100A1 Recombinant Antibody (clone PT1427) | IHC-P | Mouse IgG1, κ |
| VS3-CJ797 | Mouse Anti-S100A1 Recombinant Antibody (clone ABT-S100A1) | IF, IHC-P | Mouse IgG1, κ |
| VS3-FY1292 | Recombinant Mouse Anti-S100A1 Antibody (clone 9E8-8B8-9B4) | IHC-P | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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