Recombinant Mouse Anti-S100B Detection Antibody (clone 10F4) (CAT#: VS3-WK1667)
This antibody is a mouse recombinant antibody against human S100B. The sandwich ELISA was established using matched antibody pair 13F5 and 10F4. The clone 10F4 can be used in ELISA analysis as a detection antibody at a dilution of 1/1000. It was developed by immunization of mouse with recombinant human S100B protein and then was produced as a recombinant antibody. The isotype of this product is mouse IgG2a.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line SK-MEL-30
(Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15768/1844_C4_31_selected.jpg
(Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15768/1844_C4_31_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15768/129_E6_1_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15768/129_E6_1_red_green.jpg
Figure 3 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15768/941_H2_1_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15768/941_H2_1_red_green.jpg
Figure 4 Cerebral cortex
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73/163243_B_8_5.jpg
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73/163243_B_8_5.jpg
Figure 5 Colon
(Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73/163243_A_7_3.jpg
(Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73/163243_A_7_3.jpg
Figure 6 Soft tissue
(Peripheral nerve
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73/163243_B_1_7.jpg
(Peripheral nerve
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73/163243_B_1_7.jpg
Figure 7 Lymph node
(Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73/163243_A_8_8.jpg
(Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73/163243_A_8_8.jpg
Figure 8 RNA cell line category: Group enriched (SK-MEL-30, WM-115)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000160307-S100B
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000160307-S100B
Specifications
- Immunogen
- Recombinant human S100B protein
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human S100B
- Species Reactivity
- Human
- Clone
- 10F4
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.01% thimerosal
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- ELISA: 1/1000
Target
- Alternative Names
- S100 Calcium Binding Protein B; S-100 Protein Subunit Beta; S100 Calcium Binding Protein, Beta (Neural); S100 Calcium-Binding Protein, Beta (Neural); S-100 Calcium-Binding Protein, Beta Chain; S100 Calcium-Binding Protein B; S-100 Protein Beta Chain
- Gene ID
- 6285
- UniProt ID
- P04271
- Long Name
- S100 Calcium Binding Protein B
- Sequence Similarities
- Belongs to the S-100 family.
- Cellular Localization
- Cytoplasm, Nucleus
- Protein Refseq
- NP_006263.1
- Function
- Small zinc- and- and calcium-binding protein that is highly expressed in astrocytes and constitutes one of the most abundant soluble proteins in brain.
Recommended Products
Capture antibody
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-2226-sdAb | Recombinant Anti-Human S100B VHH Single Domain Antibody | WB, IP, ChiP, Neut, ELISA | Llama VHH |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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