Recombinant Mouse Anti-sialyl Tn Antibody (HPAB-0009LY) (CAT#: HPAB-0009LY)
This product is antibody recognizes sialyl Tn. The antibody immunoassay techniques such as: ELISA, IHC.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Specifications
- Host Species
- Mouse
- Applications
- ELISA, IHC, IF, FC, WB, IP
Product Property
- Purification
- 95% pure by SDS-PAGE
- Format
- Liquid
- Concentration
- 1 mg/ml
- Buffer
- PBS, 150 mM NaCl, 0.01% BSA, pH 6
- Preservative
- 0.05% NaN3
- Storage
- Store it under sterile conditions at -20°C upon receiving. Recommend to pack the protein into smaller quantities for optimal storage.
Applications
- Application Notes
- The sialyl Tn antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Western Blot, Immunoprecipitation.
IF: Sections were incubated with the monoclonal antibody PMH1 or TKH2 diluted in PBS/5%BSA overnight at 4°C. Sections were washed with PBS and incubated for 30 min with Texas-Red-conjugated goat anti-mouse IgM (in the case of PMH1) or Texas-Red-conjugated goat anti-mouse IgG1-specific (in the case of TKH2) diluted 1:70 in PBS/5%BSA and DAPI diluted 1:100, for 30 min in the dark.
FC: TKH2 as a culture supernatant diluted 1/2 and B72.3 at 1 μg/ml or with the biotinylated PNA and JAC lectins at 1 μg/ml and 0.25 μg/ml, respectively, in PBS containing 0.1% gelatin for 30 minutes at 4°C. After three washes with this same buffer, a 30 minute incubation with the secondary anti-mouse IgG FITC-labeled antibody (Sigma) or with FITC-labeled streptavidin was performed at 4°C.
WB: Membranes were then incubated with the mAbs TKH2 or B72.3 diluted in PBS containing 1% skim milk overnight at 4°C. After washing twice in PBS/Tween 0.05% and once in PBS, membranes were incubated for 1 hour with peroxidase-labeled anti-mouse IgG diluted in PBS containing 1% skim milk.
IP: Membranes were then incubated with the mAbs TKH2 or B72.3 diluted in PBS containing 1% skim milk overnight at 4°C. After washing twice in PBS/Tween 0.05% and once in PBS, membranes were incubated for 1 hour with peroxidase-labeled anti-mouse IgG diluted in PBS containing 1% skim milk.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
Customer Reviews and Q&As
Customer Review
Q&As
Great for Flow Cytometry
We incorporated this antibody into our flow cytometry assays to detect sialyl-Tn expression on cancer cells. The staining was bright and specific, allowing us to easily distinguish positive cells. It required minimal optimization, saving us time and resources. This antibody has enhanced our cell analysis substantially.
Enhanced ELISA Sensitivity
Using this antibody in our ELISA setup has markedly increased sensitivity. We're detecting lower concentrations of sialyl-Tn antigens than before, which is essential for our biomarker research. The assay linearity and reproducibility have also improved, making our quantitative analyses more reliable.
User-Friendly Protocols
The antibody comes with comprehensive protocols that are easy to follow. Even researchers new to this area have been able to achieve excellent results. The support materials have made integration into our existing workflows smooth, and we've appreciated the time saved.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# HPAB-0009LY, RRID: AB_3111343)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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