Recombinant Mouse Anti-sialyl Tn Antibody (HPAB-0009LY)
CAT#: HPAB-0009LY
This product is antibody recognizes sialyl Tn. The antibody immunoassay techniques such as: ELISA, IHC.
Specifications
- Host Species
- Mouse
- Applications
- ELISA, IHC, IF, FC, WB, IP
Product Property
- Purification
- 95% pure by SDS-PAGE
- Format
- Liquid
- Concentration
- 1 mg/ml
- Buffer
- PBS, 150 mM NaCl, 0.01% BSA, pH 6
- Preservative
- 0.05% NaN3
- Storage
- Store it under sterile conditions at -20°C upon receiving. Recommend to pack the protein into smaller quantities for optimal storage.
Applications
- Application Notes
- The sialyl Tn antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Western Blot, Immunoprecipitation.
IF: Sections were incubated with the monoclonal antibody PMH1 or TKH2 diluted in PBS/5%BSA overnight at 4°C. Sections were washed with PBS and incubated for 30 min with Texas-Red-conjugated goat anti-mouse IgM (in the case of PMH1) or Texas-Red-conjugated goat anti-mouse IgG1-specific (in the case of TKH2) diluted 1:70 in PBS/5%BSA and DAPI diluted 1:100, for 30 min in the dark.
FC: TKH2 as a culture supernatant diluted 1/2 and B72.3 at 1 μg/ml or with the biotinylated PNA and JAC lectins at 1 μg/ml and 0.25 μg/ml, respectively, in PBS containing 0.1% gelatin for 30 minutes at 4°C. After three washes with this same buffer, a 30 minute incubation with the secondary anti-mouse IgG FITC-labeled antibody (Sigma) or with FITC-labeled streptavidin was performed at 4°C.
WB: Membranes were then incubated with the mAbs TKH2 or B72.3 diluted in PBS containing 1% skim milk overnight at 4°C. After washing twice in PBS/Tween 0.05% and once in PBS, membranes were incubated for 1 hour with peroxidase-labeled anti-mouse IgG diluted in PBS containing 1% skim milk.
IP: Membranes were then incubated with the mAbs TKH2 or B72.3 diluted in PBS containing 1% skim milk overnight at 4°C. After washing twice in PBS/Tween 0.05% and once in PBS, membranes were incubated for 1 hour with peroxidase-labeled anti-mouse IgG diluted in PBS containing 1% skim milk.
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Great for Flow Cytometry
We incorporated this antibody into our flow cytometry assays to detect sialyl-Tn expression on cancer cells. The staining was bright and specific, allowing us to easily distinguish positive cells. It required minimal optimization, saving us time and resources. This antibody has enhanced our cell analysis substantially.
Enhanced ELISA Sensitivity
Using this antibody in our ELISA setup has markedly increased sensitivity. We're detecting lower concentrations of sialyl-Tn antigens than before, which is essential for our biomarker research. The assay linearity and reproducibility have also improved, making our quantitative analyses more reliable.
User-Friendly Protocols
The antibody comes with comprehensive protocols that are easy to follow. Even researchers new to this area have been able to achieve excellent results. The support materials have made integration into our existing workflows smooth, and we've appreciated the time saved.
Q&As
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How can the recombinant anti-sialyl-Tn antibody be used to study cancer progression in colorectal cancer research?
A: The recombinant anti-sialyl-Tn antibody specifically targets the sialyl-Tn antigen, which is overexpressed in colorectal cancer cells. By using this antibody in immunohistochemistry or flow cytometry, researchers can detect and quantify the expression levels of sialyl-Tn on tumor cells. This allows for the assessment of tumor progression, metastasis potential.
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How does the antibody's specificity enhance its use in immunoassays for ovarian cancer research?
A: The high specificity of the antibody for the sialyl-Tn antigen ensures that it binds exclusively to target molecules present on ovarian cancer cells. In immunoassays like ELISA or immunocytochemistry, this reduces background noise and increases signal clarity. As a result, researchers obtain more accurate and reliable data when measuring antigen levels or observing antigen localization within cells.
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Can this antibody be used to study the interaction between cancer cells and the extracellular matrix? How?
A: Yes, the antibody can help study how sialyl-Tn antigens on cancer cells interact with components of the extracellular matrix (ECM). By labeling these antigens, researchers can observe adhesion properties, migration through the ECM, and how these interactions facilitate invasion and metastasis. This may lead to identifying new targets to inhibit tumor progression.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# HPAB-0009LY, RRID: AB_3111343)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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