Recombinant Mouse Anti-SMARCB1 Antibody (clone 5F1-6F6-3A1)
CAT#: VS3-FY1362
This product is a recombinant mouse antibody that recognizes SMARCB1. This antibody has been reported for use in Immunohistochemistry-Paraffin. The clone 5F1-6F6-3A1 is specific for human SMARCB1. The antigen is synthetic peptide derived from human ini-1.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18248/144_B9_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows nuclear positivity.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18248/40923_A_4_8_selected.jpg
Normal Tissue
Figure 3 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18248/40923_A_4_6.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18248/40923_A_7_8.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18248/40923_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18248/40923_A_7_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18248/40923_A_7_5.jpg
Normal Tissue
Figure 8 Urinary bladder
Urothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18248/40923_A_4_5.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000099956-SMARCB1
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IHC-P
Figure 1. Recombinant Mouse Anti-SMARCB1 Antibody (clone 5F1-6F6-3A1) in IHC-P.
Immunohistochemical analysis of paraffin-embedded astrocytoma using SMARCB1 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- Synthetic peptide derived from human INI-1.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human SMARCB1
- Species Reactivity
- Human
- Clone
- 5F1-6F6-3A1
- Applications
- Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.3, 50% glycerol, 0.5% BSA
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry-Paraffin: 1/100-1/500
Target
- Alternative Names
- SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily B, Member 1; Protein Phosphatase 1, Regulatory Subunit 144; Sucrose Nonfermenting, Yeast, Homolog-Like 1; Malignant Rhabdoid Tumor Suppressor; Integrase Interactor 1 Protein; BRG1-Associated Factor 47; SNF5 Homolog; SNF5L1; BAF47; HSNF5; INI1; SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily B Member 1; SWI/SNF-Related Matrix-Associated Protein
- Gene ID
- 6598
- UniProt ID
- Q12824
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
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Recommended Dilution Buffer
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Isotype Control
- CAT
- Product Name
See other products for "SMARCB1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4029z | Mouse Anti-SMARCB1 Recombinant Antibody (clone 21H6) | WB, ELISA, IHC | Mouse IgG1, κ |
| MRO-1415-CN | Recombinant Rabbit Anti-SMARCB1 Monoclonal Antibody (JB49-18) | WB, IHC, FC | Rabbit IgG |
| MOB-0057F | Mouse Anti-SMARCB1 Recombinant Antibody (MOB-0057F) | IHC-P, IF | Mouse IgG |
| VS3-WK311 | Rabbit Anti-SMARCB1 Recombinant Antibody (clone JB49-18) | WB, ELISA | Rabbit IgG |
| VS3-WK908 | Mouse Anti-SMARCB1 Recombinant Antibody (clone ABT-INI1) | IHC-P, WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3330 | Hi-Affi™ Recombinant Rabbit Anti-SMARCB1 Monoclonal Antibody (DS3330AB) | WB, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6735 | Anti-SMARCB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6736 | Anti-Mouse SMARCB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6737 | Anti-Rat SMARCB1 Immunohistochemistry Kit | IHC |
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