Recombinant Rabbit Anti-SMYD3 Antibody (clone R03-2I7)
CAT#: VS3-FY1364
This product is a recombinant rabbit antibody that recognizes SMYD3. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The antigen is human SMYD3 synthetic peptide. The clone R03-2I7 is specific for human SMYD3.
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line RT4
Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45821/1766_D7_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45821/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12229/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45821/1042_A9_2_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/54352/139734_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/54352/139734_A_7_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/54352/139734_A_7_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/54352/139734_A_6_6.jpg
Normal Tissue
Figure 9 Skeletal muscle
Myocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/54352/139734_B_2_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/54352/139734_A_8_8.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enhanced (HMC-1, K-562)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000185420-SMYD3
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ICC
Figure 1. Recombinant Rabbit Anti-SMYD3 Antibody (clone R03-2I7) in ICC.
Immunocytochemical analysis of SMYD3 (green) in K562 using SMYD3 antibody and DAPI (blue).
WB
Figure 2. Recombinant Rabbit Anti-SMYD3 Antibody (clone R03-2I7) in WB.
Western Blot analysis of SMYD3 in Jurkat, Hela lysates using SMYD3 Antibody.
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Specifications
- Immunogen
- Human SMYD3 synthetic peptide.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human SMYD3
- Species Reactivity
- Human
- Clone
- R03-2I7
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 49 kDa; Observed MW: 49 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "SMYD3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-558 | Recombinant Human Anti-Human SMYD3 Antibody Fab Fragment, clone D339 | ELISA, IP-MS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3349 | Hi-Affi™ Recombinant Rabbit Anti-SMYD3 Monoclonal Antibody (DS3349AB) | IHC-P, WB, ICC, IF, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1413-CN | Recombinant Rabbit Anti-SMYD3 Monoclonal Antibody (JM73-63) | WB, IF, IHC, IP, FC | Rabbit IgG |
| VS3-WK611 | Rabbit Anti-SMYD3 Recombinant Antibody (clone JM73-63) | WB, ELISA | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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