Recombinant Rabbit Anti-SOX9 Detection Antibody (clone R97-6V-1) (CAT#: VS3-WK1931)
This antibody is a rabbit recombinant antibody against human SOX9. It was developed by immunization of rabbit with recombinant human SOX9 protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. R65-8T-3 and R97-6V-1 were identified as antibody pair for sandwich ELISA. As a detection antibody, the clone R97-6V-1 can be used in ELISA analysis to identify human SOX9.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22456/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22456/if_selected.jpg
Figure 2 Salivary gland.
(High expression in salivary gland)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68240/159895_B_1_1_selected.jpg
(High expression in salivary gland)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68240/159895_B_1_1_selected.jpg
Figure 3 Cerebral cortex
(Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68240/159895_B_7_5.jpg
(Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68240/159895_B_7_5.jpg
Figure 4 Colon
(Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity: 75%-25% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity: 75%-25% Mucosal lymphoid cells Staining: Medium Intensity: Strong Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68240/159895_A_7_3.jpg
(Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity: 75%-25% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity: 75%-25% Mucosal lymphoid cells Staining: Medium Intensity: Strong Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68240/159895_A_7_3.jpg
Figure 5 Liver
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68240/159895_A_8_4.jpg
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68240/159895_A_8_4.jpg
Figure 6 Kidney
(Bowman's capsule Staining: High Intensity: Strong Quantity:>75% Collecting ducts Staining: High Intensity: Strong Quantity: 75%-25% Distal tubules Staining: Medium Intensity: Strong Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68240/159895_A_7_5.jpg
(Bowman's capsule Staining: High Intensity: Strong Quantity:>75% Collecting ducts Staining: High Intensity: Strong Quantity: 75%-25% Distal tubules Staining: Medium Intensity: Strong Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68240/159895_A_7_5.jpg
Figure 7 Testis
(Sertoli cells Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68240/159895_A_5_6.jpg
(Sertoli cells Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68240/159895_A_5_6.jpg
Figure 8 RNA cell line category: Cell line enhanced (AN3-CA, Hep G2, OE19, U-251 MG)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000125398-SOX9
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000125398-SOX9
Specifications
- Immunogen
- Recombinant human SOX9 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human SOX9
- Species Reactivity
- Human
- Clone
- R97-6V-1
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- SRY-Box 9; Campomelic Dysplasia, Autosomal Sex-Reversal; SRY (Sex-Determining Region Y)-Box 9 Protein; SRY (Sex Determining Region Y)-Box 9; SRY (Sex Determining Region Y)-Box9; SRY-Related HMG-Box, Gene 9; Transcription Factor SOX-9
- Gene ID
- 6662
- UniProt ID
- P48436
- Long Name
- SRY-Box 9
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Acetylated; acetylation impairs nuclear localization and ability to transactivate expression of target genes. Deacetylated by SIRT1.
Phosphorylation at Ser-64 and Ser-211 by PKA increases transcriptional activity and may help delay chondrocyte maturation downstream of PTHLH/PTHrP signaling. Phosphorylation at either Ser-64 or Ser-211 is required for sumoylation, but phosphorylation is not dependent on sumoylation. Phosphorylated on tyrosine residues; tyrosine dephosphorylation by PTPN11/SHP2 blocks SOX9 phosphorylation by PKA and subsequent SUMOylation.
Ubiquitinated; ubiquitination leads to proteasomal degradation and is negatively regulated by DDRGK1.
Sumoylated; phosphorylation at either Ser-64 or Ser-211 is required for sumoylation. Sumoylation is induced by BMP signaling pathway.
- Protein Refseq
- NP_000337.1
- Function
- Transcription factor that plays a key role in chondrocytes differentiation and skeletal development.
Specifically binds the 5'-ACAAAG-3' DNA motif present in enhancers and super-enhancers and promotes expression of genes important for chondrogenesis, including cartilage matrix protein-coding genes COL2A1, COL4A2, COL9A1, COL11A2 and ACAN, SOX5 and SOX6.
Recommended Products
Capture antibody
Isotype Control
Secondary Antibody
Related Resources
cAMP Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "SOX9"
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Please select product type
IgG Antibody Products
Rabbit Monoclonal Antibody Products
IgM Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3882z | Mouse Anti-SOX9 Recombinant Antibody (clone 15C2) | WB, ICC, IF, ELISA | Mouse IgG1 |
| MRO-1427-CN | Recombinant Rabbit Anti-SOX9 Monoclonal Antibody (CBACN-524) | WB, IF, IHC, IP, FC | Rabbit IgG |
| ZG-0122U | Rabbit Anti-SOX9 Recombinant Antibody (clone 5H12) | ELISA, IHC | Rabbit IgG |
| VS3-CJ981 | Mouse Anti-SOX9 Recombinant Antibody (VS3-CJ981) | WB, IHC | Mouse IgG2b |
| VS3-CJ982 | Rabbit Anti-SOX9 Recombinant Antibody (VS3-CJ982) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
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