Recombinant Mouse Anti-SPN Antibody (clone 4G4-7C7-8H8) (CAT#: VS3-FY1381)

• Immunogen
• Synthetic peptide derived from human CD43.

• Host Species
• Mouse

• Type
• Mouse IgG1

• Specificity
• Human SPN

• Species Reactivity
• Human

• Clone
• 4G4-7C7-8H8

• Applications
• Immunohistochemistry-Paraffin

• Conjugate
• Unconjugated

• Purification
• Affinity Chromatography

• Purity
• >95% as determined by SDS-PAGE

• Buffer
• PBS, pH 7.3, 50% glycerol, 0.5% BSA

• Preservative
• 0.02% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Immunohistochemistry-Paraffin: 1/100-1/500

• Alternative Names
• Sialophorin; Sialophorin (GpL115, Leukosialin, CD43); Leukocyte Sialoglycoprotein; Galactoglycoprotein; Leukosialin; GALGP

• Gene ID
• 6693

• UniProt ID
• P16150

• Cellular Localization
• Cell projection, Membrane, Nucleus

• Post Translation Modifications
• Glycosylated; has a high content of sialic acid and O-linked carbohydrate structures.
  Phosphorylation at Ser-355 is regulated by chemokines, requires its association with ERM proteins (EZR, RDX and MSN) and is essential for its function in the regulation of T-cell trafficking to lymph nodes.
  Has a high content of sialic acid and O-linked carbohydrate structures.
  Cleavage by CTSG releases its extracellular domain and triggers its intramembrane proteolysis by gamma-secretase releasing the CD43 cytoplasmic tail chain (CD43-ct) which translocates to the nucleus.
  CD43 cytoplasmic tail: Sumoylated.

• Protein Refseq
• NP_001025459.1; NP_003114.1

• Function
• Predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. Positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN) (By similarity).
  Negatively regulates Th2 cell differentiation and predisposes the differentiation of T-cells towards a Th1 lineage commitment. Promotes the expression of IFN-gamma by T-cells during T-cell receptor (TCR) activation of naive cells and induces the expression of IFN-gamma by CD4+ T-cells and to a lesser extent by CD8+ T-cells (PubMed:18036228).
  Plays a role in preparing T-cells for cytokine sensing and differentiation into effector cells by inducing the expression of cytokine receptors IFNGR and IL4R, promoting IFNGR and IL4R signaling and by mediating the clustering of IFNGR with TCR (PubMed:24328034).
  Acts as a major E-selectin ligand responsible for Th17 cell rolling on activated vasculature and recruitment during inflammation. Mediates Th17 cells, but not Th1 cells, adhesion to E-selectin. Acts as a T-cell counter-receptor for SIGLEC1 (By similarity).
  CD43 cytoplasmic tail:
  Protects cells from apoptotic signals, promoting cell survival.

Secondary Antibody

• 2ND-047CQ
• Goat Anti-Mouse IgG1 Antibody, R-PE

• 2RD-A1460-YJ
• Goat Anti-Mouse IgG1 Secondary antibody

Isotype Control

• MOB-065CQ
• Mouse IgG1 Isotype Control Antibody

Reconstitution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

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