Recombinant Mouse Anti-SPN Antibody (clone 4G4-7C7-8H8)
CAT#: VS3-FY1381
This product is a recombinant mouse antibody that recognizes SPN. This antibody has been reported for use in Immunohistochemistry-Paraffin. The clone 4G4-7C7-8H8 is specific for human SPN. The antigen is synthetic peptide derived from human cd43.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line REH
Immunofluorescent staining of human cell line REH shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55244/1817_E10_2_selected.jpg
Normal Tissue
Figure 2 Lung
Macrophages
Staining:
High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55244/134325_A_2_4.jpg
Normal Tissue
Figure 3 Lymph node
Non-germinal center cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55244/134325_A_8_8.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (HEL, HL-60, HMC-1, MOLT-4, REH, THP-1, U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000197471-SPN
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IHC-P
Figure 1. Recombinant Mouse Anti-SPN Antibody (clone 4G4-7C7-8H8) in IHC-P.
Immunohistochemical analysis of paraffin-embedded anaplastic large cell lymphoma using CD43 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- Synthetic peptide derived from human CD43.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human SPN
- Species Reactivity
- Human
- Clone
- 4G4-7C7-8H8
- Applications
- Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.3, 50% glycerol, 0.5% BSA
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry-Paraffin: 1/100-1/500
Target
- Alternative Names
- Sialophorin; Sialophorin (GpL115, Leukosialin, CD43); Leukocyte Sialoglycoprotein; Galactoglycoprotein; Leukosialin; GALGP
- Gene ID
- 6693
- UniProt ID
- P16150
- Cellular Localization
- Cell projection, Membrane, Nucleus
- Post Translation Modifications
- Glycosylated; has a high content of sialic acid and O-linked carbohydrate structures.
Phosphorylation at Ser-355 is regulated by chemokines, requires its association with ERM proteins (EZR, RDX and MSN) and is essential for its function in the regulation of T-cell trafficking to lymph nodes.
Has a high content of sialic acid and O-linked carbohydrate structures.
Cleavage by CTSG releases its extracellular domain and triggers its intramembrane proteolysis by gamma-secretase releasing the CD43 cytoplasmic tail chain (CD43-ct) which translocates to the nucleus.
CD43 cytoplasmic tail: Sumoylated.
- Protein Refseq
- NP_001025459.1; NP_003114.1
- Function
- Predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. Positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN) (By similarity).
Negatively regulates Th2 cell differentiation and predisposes the differentiation of T-cells towards a Th1 lineage commitment. Promotes the expression of IFN-gamma by T-cells during T-cell receptor (TCR) activation of naive cells and induces the expression of IFN-gamma by CD4+ T-cells and to a lesser extent by CD8+ T-cells (PubMed:18036228).
Plays a role in preparing T-cells for cytokine sensing and differentiation into effector cells by inducing the expression of cytokine receptors IFNGR and IL4R, promoting IFNGR and IL4R signaling and by mediating the clustering of IFNGR with TCR (PubMed:24328034).
Acts as a major E-selectin ligand responsible for Th17 cell rolling on activated vasculature and recruitment during inflammation. Mediates Th17 cells, but not Th1 cells, adhesion to E-selectin. Acts as a T-cell counter-receptor for SIGLEC1 (By similarity).
CD43 cytoplasmic tail:
Protects cells from apoptotic signals, promoting cell survival.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0285MZ | Recombinant Mouse Anti-Human Sialophorin Antibody (clone EF-T2) | IHC | Mouse antibody |
| HPAB-3177LY | Human Anti-SPN Recombinant Antibody (HPAB-3177LY) | Inhib, IHC, FC, WB | Humanized IgG |
| HPAB-3178LY | Human Anti-SPN Recombinant Antibody (HPAB-3178LY) | Inhib, IHC, FC, WB | Humanized IgG |
| HPAB-3179LY | Human Anti-SPN Recombinant Antibody (HPAB-3179LY) | Inhib, IHC, FC, WB | Humanized IgG |
| HPAB-3180LY | Human Anti-SPN Recombinant Antibody (HPAB-3180LY) | Inhib, IHC, FC, WB | Humanized IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1980CQ | Recombinant Mouse Anti-Spn Antibody (L11) | BL, IP, FC, IHC | IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3391 | Hi-Affi™ Recombinant Rabbit Anti-SPN Monoclonal Antibody (DS3391AB) | IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-3177LY-S(P) | Human Anti-SPN Recombinant Antibody; scFv Fragment (HPAB-3177LY-S(P)) | Inhib, IHC, FC, WB | Human scFv |
| HPAB-3178LY-S(P) | Human Anti-SPN Recombinant Antibody; scFv Fragment (HPAB-3178LY-S(P)) | Inhib, IHC, FC, WB | Human scFv |
| HPAB-3179LY-S(P) | Human Anti-SPN Recombinant Antibody; scFv Fragment (HPAB-3179LY-S(P)) | Inhib, IHC, FC, WB | Human scFv |
| HPAB-3180LY-S(P) | Human Anti-SPN Recombinant Antibody; scFv Fragment (HPAB-3180LY-S(P)) | Inhib, IHC, FC, WB | Human scFv |
| HPAB-3181LY-S(P) | Human Anti-SPN Recombinant Antibody; scFv Fragment (HPAB-3181LY-S(P)) | Inhib, IHC, FC, WB | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-3177LY-F(E) | Human Anti-SPN Recombinant Antibody; Fab Fragment (HPAB-3177LY-F(E)) | Inhib, IHC, FC, WB | Humanized Fab |
| HPAB-3178LY-F(E) | Human Anti-SPN Recombinant Antibody; Fab Fragment (HPAB-3178LY-F(E)) | Inhib, IHC, FC, WB | Humanized Fab |
| HPAB-3179LY-F(E) | Human Anti-SPN Recombinant Antibody; Fab Fragment (HPAB-3179LY-F(E)) | Inhib, IHC, FC, WB | Humanized Fab |
| HPAB-3180LY-F(E) | Human Anti-SPN Recombinant Antibody; Fab Fragment (HPAB-3180LY-F(E)) | Inhib, IHC, FC, WB | Humanized Fab |
| HPAB-3181LY-F(E) | Human Anti-SPN Recombinant Antibody; Fab Fragment (HPAB-3181LY-F(E)) | Inhib, IHC, FC, WB | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC806 | AbPlus™ Anti-SPN Magnetic Beads (VS-0724-YC806) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY203 | CytoStream™ Mouse Anti-SPN Recombinant Antibody (VS-0225-XY203) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY30 | Human Anti-SPN scFv-Fc Chimera (VS-0325-FY30) | ELISA, FC | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC504 | Recombinant Anti-SPN Vesicular Antibody, EV Displayed (VS-0425-YC504) | ELISA, FC, Inhib, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6864 | Anti-SPN Immunohistochemistry Kit | IHC | |
| VS-0525-XY6865 | Anti-Rat SPN Immunohistochemistry Kit | IHC |
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