Recombinant Rabbit Anti-SYP Antibody (clone R06-2A2)
CAT#: VS3-FY1421
This product is a recombinant rabbit antibody that recognizes SYP. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence. The clone R06-2A2 is specific for human, rat SYP. The antigen is synthetic peptide of human synaptophysin.
Gene Expression
Tested Data
Normal Tissue
Figure 1 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55505/125080_B_8_5.jpg
Normal Tissue
Figure 2 Duodenum
Goblet cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Paneth cells
Staining: High
Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55505/125080_A_9_2.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enhanced (K-562, SCLC-21H, SH-SY5Y)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000102003-SYP/subcellular
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ICC
Figure 1. Recombinant Rabbit Anti-SYP Antibody (clone R06-2A2) in ICC.
Immunocytochemical analysis of synaptophysin (green) in 3T3 using synaptophysin antibody and DAPI (blue).
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Specifications
- Immunogen
- Synthetic peptide of human synaptophysin.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Rat SYP
- Species Reactivity
- Human, Rat
- Clone
- R06-2A2
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 34 kDa; Observed MW: 38 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- SYP; Synaptophysin; Major synaptic vesicle protein p38
- Gene ID
- 6855
- UniProt ID
- P08247
- Sequence Similarities
- Belongs to the synaptophysin/synaptobrevin family.
- Cellular Localization
- Cytoplasmic vesicle, Membrane, Synapse, Synaptosome
- Post Translation Modifications
- Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
Phosphorylated by SRC.
- Protein Refseq
- NP_003170.1
- Function
- Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "SYP"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1588z | Mouse Anti-SYP Recombinant Antibody (clone 23G4) | WB, IP | Mouse IgM, κ |
| MRO-1461-CN | Recombinant Mouse Anti-SYP Monoclonal Antibody (A1-7) | WB, IF, IHC, FC | Mouse IgG1 |
| MRO-1462-CN | Recombinant Rabbit Anti-SYP Monoclonal Antibody (SJ26-85) | WB, IF, IHC | Rabbit IgG |
| VS-0223-WK69 | Rabbit Anti-SYP Recombinant Antibody (VS-0223-WK69) | WB, IP, ICC, IHC, IHC-P | Rabbit IgG |
| VS-0223-WK70 | Guinea pig Anti-SYP Recombinant Antibody (VS-0223-WK70) | WB, ICC, IHC, IHC-P | Guinea pig IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-494CT | Anti-Human Synaptophysin Recombinant Antibody (C1-3) | ELISA, FC | Human antibody |
| TAB-494CT-S(P) | Anti-Human Synaptophysin Recombinant Antibody scFv Fragment (C1-3) | ELISA, FC | Human antibody |
| TAB-494CT-F(E) | Anti-Human Synaptophysin Recombinant Antibody Fab Fragment (C1-3) | ELISA, FC | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3483 | Hi-Affi™ Recombinant Rabbit Anti-SYP Monoclonal Antibody (DS3483AB) | IHC-FoFr, IHC-P, WB | IgG |
| MOR-4514 | Hi-Affi™ Recombinant Rabbit Anti-SYP Monoclonal Antibody (TH22DS) | WB, IF, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-1544CQ | Mouse Anti-SYP Recombinant Antibody (clone SP15) | WB, ELISA, IHC | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC136 | Recombinant Anti-SYP Vesicular Antibody, EV Displayed (VS-0425-YC136) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7053 | Anti-SYP Immunohistochemistry Kit | IHC | |
| VS-0525-XY7054 | Anti-Rat SYP Immunohistochemistry Kit | IHC | |
| VS-0525-XY7055 | Anti-Mouse SYP Immunohistochemistry Kit | IHC |
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