Recombinant Anti-TGFA Vesicular Antibody, EV Displayed (VS-0425-YC553) (CAT#: VS-0425-YC553)
The Recombinant Anti-TGFA Vesicular Antibody, EV Displayed (VS-0425-YC553) is an antibody-displaying extracellular vesicle (Ab-EV). The product combines the benefits of both extracellular vesicle (EV) and antibody (Ab) which can guide the decorated EVs to TGFA-expressed cells or tissues. TGFA is a growth factor involved in cell proliferation through EGFR activation. It may act as a transmembrane or soluble ligand, with associations to cancer.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression

Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/if_selected.jpg

Figure 2 IHC staining of human rectum
(Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/ihc_selected.jpg
(Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/ihc_selected.jpg

Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/476_C8_2_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/476_C8_2_blue_red_green.jpg

Figure 4 Cerebral cortex
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/94418_B_9_5.jpg
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/94418_B_9_5.jpg

Figure 5 Colon
(Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/94418_A_9_3.jpg
(Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/94418_A_9_3.jpg

Figure 6 Liver
(Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/94418_A_9_4.jpg
(Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/94418_A_9_4.jpg

Figure 7 Kidney
(Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/94418_A_9_5.jpg
(Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/94418_A_9_5.jpg

Figure 8 Testis
(Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/94418_A_4_6.jpg
(Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/94418_A_4_6.jpg

Figure 9 Lymph node
(Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/94418_A_9_8.jpg
(Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/94418_A_9_8.jpg

Figure 10 RNA cell line category: Cell line enhanced (A-431, CAPAN-2, HBEC3-KT, OE19, RPTEC TERT1, U-87 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000163235-TGFA
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000163235-TGFA
Recombinant Antibody
- Application
- ELISA, FC, Neut, Cell-uptake
- Product Type
- Ab-Fc-EVs
- Antibody Quantification (Ab/EV)
- ~100 Ab/EV
- Target
- TGFA
- Host Animal
- Mouse
- Antibody Isotype
- IgG
- Species Reactivity
- Human
- Expression Cell
- Mammalian cell
Engineered EVs
- EV-sorting domain
- CD63
- Fc-binding domain
- Protein A
- EV Size
- 30~150 nm
- Producing Cell
- HEK293F
- Isolation Method
- Gradient centrifugation
- Purification
- qEV size exclusion chromatography
- Concentration
- 1 x 10¹⁰
- Size
- 1 mL
- Buffer
- PBS
- Storage
- Store at -80°C for 12 months
Target
- Full Name
- Transforming growth factor alpha
- Molecular Function
- Growth factor, Mitogen
- Cellular Localization
- Vesicles
- Introduction
- This gene encodes a growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
- Gene ID
- 7039
- UniProt ID
- P01135
Related Resources
Downloads
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IHC Kits & Antibodies
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MOB-1199z | Mouse Anti-TGFA Recombinant Antibody (clone 12B8) | WB, IF, IP | Mouse IgG1 |
MOB-2017CT | Recombinant Mouse anti-Human TGFA Monoclonal antibody (Q/U2) | ELISA, ICC, IHC-Fr, IHC-P | |
HPAB-0544-CN | Mouse Anti-TGFA Recombinant Antibody (HPAB-0544-CN) | WB, ELISA, FC, IHC, IP, Neut | Mouse IgG1, κ |
HPAB-0545-CN | Mouse Anti-TGFA Recombinant Antibody (HPAB-0545-CN) | WB, ELISA, FC, IHC, IP, Neut | Mouse IgG1, κ |
HPAB-0546-CN | Mouse Anti-TGFA Recombinant Antibody (HPAB-0546-CN) | WB, ELISA, FC, IHC, IP, Neut | Mouse IgG2a, κ |
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