Recombinant Rabbit Anti-Thbd Antibody (clone R02-1G1) (CAT#: VS3-FY1449)
This product is a recombinant rabbit antibody that recognizes Thbd. This antibody has been reported for use in Western Blot. The clone R02-1G1 is specific for mouse, rat Thbd. The antigen is mouse thrombomodulin recombinant protein.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2982/29_E4_2_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2982/29_E4_2_selected.jpg
Figure 2 IHC staining of human placenta
(Immunohistochemical staining of human placenta shows strong membranous positivity in trophoblastic cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2982/ihc_selected.jpg
(Immunohistochemical staining of human placenta shows strong membranous positivity in trophoblastic cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2982/ihc_selected.jpg
Figure 3 IHC staining of human placenta
(Immunohistochemical staining of human placenta shows luminal positivity.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2425/ihc_selected.jpg
(Immunohistochemical staining of human placenta shows luminal positivity.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2425/ihc_selected.jpg
Figure 4 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2982/30_E4_1_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2982/30_E4_1_red_green.jpg
Figure 5 Colon
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2425/6389_A_7_3.jpg
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2425/6389_A_7_3.jpg
Figure 6 Placenta
(Trophoblastic cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2425/6389_A_3_7.jpg
(Trophoblastic cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2425/6389_A_3_7.jpg
Figure 7 RNA cell line category: Cell line enhanced (ASC diff, CAPAN-2, EFO-21, HaCaT, hTCEpi, OE19, THP-1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000178726-THBD
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000178726-THBD
Specifications
- Immunogen
- mouse thrombomodulin recombinant protein.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Mouse, Rat Thbd
- Species Reactivity
- Mouse, Rat
- Clone
- R02-1G1
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- Calculated MW: 62 kDa; Observed MW: 95 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Thrombomodulin; CD141 Antigen; Fetomodulin; THRM; TM; THPH12; AHUS6; BDCA3; CD141
- Gene ID
- 7056
- UniProt ID
- P07204
Recommended Products
Secondary Antibody
Isotype Control
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Single Domain Antibody Products
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Flow Cytometry (FC) related Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-1329-VHH | Recombinant Anti-Mouse Thbd VHH Single Domain Antibody | IHC, ICC, FC, FA, FUNC | Llama VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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