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TRIM28
Recombinant Rabbit Anti-TRIM28 Antibody (clone R07-5E6)

Recombinant Rabbit Anti-TRIM28 Antibody (clone R07-5E6) (CAT#: VS3-FY1488)

Name: Recombinant Rabbit Anti-TRIM28 Antibody (clone R07-5E6)
SKU: VS3-FY1488
Rating: 5 (1 reviews)

This product is a recombinant rabbit antibody that recognizes TRIM28. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence. The clone R07-5E6 is specific for human, mouse, rat TRIM28. The antigen is synthetic peptide of human kap1.

Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1. Recombinant Rabbit Anti-TRIM28 Antibody (clone R07-5E6) in WB.

Figure 1. Recombinant Rabbit Anti-TRIM28 Antibody (clone R07-5E6) in WB.

Western Blot analysis of KAP1 in K562, C6, 3T3, Hela lysates using KAP1 Antibody.

ICC

Figure 2. Recombinant Rabbit Anti-TRIM28 Antibody (clone R07-5E6) in ICC.

Figure 2. Recombinant Rabbit Anti-TRIM28 Antibody (clone R07-5E6) in ICC.

Immunocytochemical analysis of KAP1 (green) in Hela using KAP1 antibody and DAPI (blue).

Subcellular Location

Figure 1 IF staining of human cell line BJ
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/1132_B11_2_selected.jpg

Figure 2 IF staining of human cell line BJ
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10066/if_selected.jpg

Figure 3 IF staining of human cell line BJ
(Immunohistochemical staining of human fallopian tube shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_A_9_7_selected.jpg

Normal Tissue

Figure 4 Cerebral cortex
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_B_7_5.jpg

Figure 5 Colon
(Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Fibroblasts
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_A_7_3.jpg

Figure 6 Liver
(Cholangiocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_A_7_4.jpg

Figure 7 Kidney
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_A_7_5.jpg

Figure 8 Testis
(Leydig cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Peritubular cells
Staining: High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining: High
Intensity: Strong
Quantity: 75%-25%
Sertoli cells
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_A_4_6.jpg

Figure 9 Skin
(Fibroblasts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Keratinocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Langerhans
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Melanocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_B_7_1.jpg

Figure 10 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_A_8_8.jpg

RNA Expression

Figure 11 RNA cell line category: Cell line enhanced (HEK93)
(Cell lines ordered by descending RNA expression order.)
*

Figure 11 RNA cell line category: Cell line enhanced (HEK93)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000130726-TRIM28

Datasheet

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Synthetic peptide of human KAP1.

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human, Mouse, Rat TRIM28

Species Reactivity
Human, Mouse, Rat

Clone
R07-5E6

Applications
Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence

Conjugate
Unconjugated

MW
Calculated MW: 89 kDa; Observed MW: 100 kDa

Product Property

Purification
Affinity Purified

Purity
>95% as determined by SDS-PAGE

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200

Target

Alternative Names
Tripartite Motif Containing 28; Protein Phosphatase 1, Regulatory Subunit 157; RING-Type E3 Ubiquitin Transferase TIF1-Beta; E3 SUMO-Protein Ligase TRIM28; KRAB-Interacting Protein 1; Nuclear Corepressor KAP-1; RING Finger Protein 96; TIF1-Beta; KRIP-1; KAP-1; RNF96; TIF1B

Gene ID
10155

UniProt ID
Q13263

Cellular Localization
Nucleus

Secondary Antibody

Isotype Control

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "TRIM28"

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Please select product type

IgG Antibody Products Single Domain Antibody Products Rabbit Monoclonal Antibody Products Antibody Magnetic Beads

CAT	Product Name	Application	Type
DrMAB-622	Human Anti-TRIM28 Recombinant Antibody (clone D530)	ELISA, IP-MS	Human IgG
MRO-2242-CN	Rabbit Anti-TRIM28 Polyclonal Antibody (MRO-2242-CN)	WB, IF, FC	Rabbit IgG
VS4-CJ141	Recombinant Mouse Anti-TRIM28 Antibody (VS4-CJ141)	ELISA, WB, IHC, ICC	Mouse IgG1
VS3-WK2064	Rabbit Anti-TRIM28 (pS824) Recombinant Antibody (clone R16-8K-7)	WB, IP	Rabbit IgG
VS3-FY2907	Mouse Anti-TRIM28 Recombinant Antibody (VS3-FY2907)	WB, IHC, ICC, FC, ELISA	Mouse IgG

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