Recombinant Rabbit Anti-TSG101 Antibody (clone R01-4D9)
CAT#: VS3-FY1491
This product is a recombinant rabbit antibody that recognizes TSG101. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence. The clone R01-4D9 is specific for human, mouse, rat TSG101. The antigen is human tsg101 synthetic peptide.
Tested Data
Gene Expression
WB
Figure 1. Recombinant Rabbit Anti-TSG101 Antibody (clone R01-4D9) in WB.
Western Blot analysis of TSG101 in K562, rat brain, C6, 3T3, Hela lysates using TSG101 Antibody.
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6161/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human rectum
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6161/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4283/11885_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4283/11885_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6161/19481_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4283/11885_A_7_5.jpg
Normal Tissue
Figure 7 Urinary bladder
Urothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4283/11885_A_5_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4283/11885_A_6_6.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4283/11885_A_9_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000074319-TSG101/subcellular
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Specifications
- Immunogen
- Human TSG101 synthetic peptide.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat TSG101
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-4D9
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 44 kDa; Observed MW: 44 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
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Customer Review
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Excellent Western Blot
Clone R01-4D9 is a fantastic antibody for detecting TSG101. We used it in Western blot and observed a specific band at 44 kDa. It works across human, mouse, and rat species, which is essential for our comparative studies on tumor susceptibility genes.
Precise IHC Staining
We validated this rabbit recombinant antibody for immunohistochemistry on paraffin sections. At a 1:50 dilution, it provided clear and precise staining of TSG101. The antibody is affinity purified and supplied in a stable buffer, making it a reliable reagent for our histology needs.
Q&As
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What applications is this TSG101 antibody reported for?
A: This antibody (clone R01-4D9) has been reported for use in Western Blot (WB), Immunohistochemistry-Frozen (IHC-Fr), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), and Immunofluorescence (IF).
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What is the immunogen and species reactivity?
A: The antibody was generated against a human TSG101 synthetic peptide. It is a Recombinant Rabbit Antibody that is specific for Human, Mouse, and Rat TSG101.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "TSG101"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1011 | Recombinant Anti-human TSG101 Antibody | WB, IHC, FuncS | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1011-F(E) | Recombinant Anti-human TSG101 Antibody Fab Fragment | FC, WB, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1011-S(P) | Recombinant Anti-human TSG101 Antibody scFv Fragment | ELISA, WB, Neut, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-1011 | Recombinant Human Anti-human TSG101 Antibody | IF, FC, Biosensors, FuncS | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-1011-F(E) | Recombinant Human Anti-human TSG101 Antibody Fab Fragment | IF, ELISA, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-1011-S(P) | Recombinant Human Anti-human TSG101 Antibody scFv Fragment | ELISA, WB, FC, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-650CL | Human Anti-TSG101 Recombinant Antibody (TAB-650CL) | ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2040CT | Recombinant Mouse anti-Human TSG101 Monoclonal antibody (6C8) | ICC, IF, IHC-P, WB, sELISA |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3704 | Rabbit Anti-TSG101 Recombinant Antibody (clone DS3704AB) | WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1535-CN | Rabbit Anti-TSG101 Recombinant Antibody (clone CBACN-563) | WB, IF, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0259U | Mouse Anti-TSG101 Recombinant Antibody (clone 6F4D11) | ELISA, WB, IF, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC611 | AbPlus™ Anti-TSG101 Magnetic Beads (VS-0724-YC611) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY517 | Mouse Anti-NHP TSG101 Recombinant Antibody (clone 4A10) | WB, IHC, ICC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2380 | Anti-TSG101 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7570 | Anti-Human TSG101 Immunohistochemistry Kit | IHC |
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