Recombinant Rabbit Anti-TUBB3 Antibody (clone R02-4G7)
CAT#: VS3-FY1497
This product is a recombinant rabbit antibody that recognizes TUBB3. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R02-4G7 is specific for human, mouse, rat TUBB3. The antigen is synthetic peptide of human β iii tubulin.
Gene Expression
Tested Data
RNA Expression
Figure 1 RNA cell line category: Cell line enhanced (A549, SCLC-21H, SH-SY5Y)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000258947-TUBB3
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WB
Figure 1. Recombinant Rabbit Anti-TUBB3 Antibody (clone R02-4G7) in WB.
Western Blot analysis of β III tubulin in rat brain using HeLa lysate of β III tubulin.
IHC-P
Figure 2. Recombinant Rabbit Anti-TUBB3 Antibody (clone R02-4G7) in IHC-P.
Immunohistochemical analysis of paraffin-embedded human cholangiocarcinoma.
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Specifications
- Immunogen
- Synthetic peptide of human β III tubulin.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat TUBB3
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R02-4G7
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 50 kDa; Observed MW: 50 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "TUBB3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-408 | Human Anti-TUBB3 Recombinant Antibody (clone YC094); Fab Fragment | WB, ELISA, FuncS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-408 | Human Anti-TUBB3 Recombinant Antibody (clone YC094); scFv Fragment | WB, ELISA, FuncS | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0539MZ | Recombinant Mouse Anti-Human TUBB3 Antibody (clone UV-30) | IHC-P | Mouse antibody |
| MOB-109CQ | Mouse Anti-TUBB3 Recombinant Antibody (clone YC153) | ELISA, WB, IHC, ICC, IP | Mouse IgG1 |
| MRO-1689-CN | Rabbit Anti-TUBB3 Polyclonal Antibody (MRO-1689-CN) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-1693-CN | Rabbit Anti-TUBB3 Polyclonal Antibody (MRO-1693-CN) | WB, IHC, FC | Rabbit IgG |
| ZG-0221C | Mouse Anti-TUBB3 Recombinant Antibody (ZG-0221C) | WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0739MZ | Chicken Anti-TUBB3 Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3723 | Rabbit Anti-TUBB3 Recombinant Antibody (clone DS3723AB) | WB, FC, IP, IHC | Rabbit IgG |
| MOR-0072-FY | Rabbit Anti-TUBB3 Recombinant Antibody (clone AFY0043) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1458 | AbPlus™ Anti-TUBB3 Magnetic Beads (VS-0724-YC1458) | IP, Protein Purification |
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