Recombinant Rabbit Anti-UCHL1 Antibody (clone R03-6E5)
CAT#: VS3-FY1520
This product is a recombinant rabbit antibody that recognizes UCHL1. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The antigen is synthetic peptide of human PGP9.5. The clone R03-6E5 is specific for human, mouse, rat UCHL1.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5993/1902_A11_2_selected.jpg
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WB
Figure 1. Recombinant Rabbit Anti-UCHL1 Antibody (clone R03-6E5) in WB.
Western Blot analysis of PGP9.5 in rat brain lysates using a PGP9.5 antibody.
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Specifications
- Immunogen
- Synthetic peptide of human PGP9.5.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat UCHL1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R03-6E5
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 25 kDa; Observed MW: 25 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Ubiquitin C-Terminal Hydrolase L1; Ubiquitin Carboxyl-Terminal Esterase L1 (Ubiquitin Thiolesterase); Neuron Cytoplasmic Protein 9.5; Ubiquitin Thioesterase L1; Ubiquitin Thiolesterase; PGP 9.5; Uch-L1; PGP9.5; Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1; Epididymis Secretory Protein Li 53
- Gene ID
- 7345
- UniProt ID
- P09936
- Sequence Similarities
- Belongs to the peptidase C12 family.
- Cellular Localization
- Cytoplasm, Endoplasmic reticulum, Membrane
- Post Translation Modifications
- O-glycosylated.
- Protein Refseq
- NP_004172.2
- Function
- Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "UCHL1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1897MZ | Recombinant Mouse Anti-Human UCHL1 Antibody (clone 20B2) | IHC-Fr, IHC-P, WB | Mouse antibody |
| VS3-QX1215 | Mouse Anti-UCHL1 Recombinant Antibody (clone 7B8-G5-E7) | WB, ICC | Mouse IgG2b |
| VS3-QX1216 | Mouse Anti-UCHL1 Recombinant Antibody (clone 696CT2.1.4) | WB, IHC | Mouse IgG1 |
| VS-0723-WK91 | Rabbit Anti-UCHL1 Recombinant Antibody (VS-0723-WK91) | ELISA | Rabbit IgG |
| VS3-WK1908 | Recombinant Rabbit Anti-UCHL1 Capture Antibody (clone R18-1C-8) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0615MZ | Chicken Anti-UCHL1 Polyclonal IgY | WB | Chicken antibody |
| BRD-0800MZ | Chicken Anti-PGP9.5 Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3771 | Rabbit Anti-UCHL1 Recombinant Antibody (clone DS3771AB) | WB, IP, IHC-P, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY270 | AbPlus™ Anti-UCHL1 Magnetic Beads (JF93-08) | IP, Protein Purification | |
| VS-0724-YC1162 | AbPlus™ Anti-Uchl1 Magnetic Beads (VS-0724-YC1162) | IP, Protein Purification | |
| VS-0724-YC1164 | AbPlus™ Anti-UCHL1 Magnetic Beads (VS-0724-YC1164) | IP, Protein Purification | |
| VS-0724-YC1237 | AbPlus™ Anti-Uchl1 Magnetic Beads (VS-0724-YC1237) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2427 | Anti-UCHL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7722 | Anti-Mouse UCHL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7723 | Anti-Rat UCHL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7721 | Anti-Human UCHL1 Immunohistochemistry Kit | IHC |
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