Recombinant Rabbit Anti-UCHL1 Detection Antibody (clone R37-3A-8)
CAT#: VS3-WK1909
This antibody is a rabbit recombinant antibody against human UCHL1. It was developed by immunization of rabbit with recombinant human UCHL1 protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. R18-1C-8 and R37-3A-8 were identified as antibody pair for sandwich ELISA. As a detection antibody, the clone R37-3A-8 can be used in ELISA analysis to identify human UCHL1.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5993/1902_A11_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/665_E11_1_selected.jpg
Normal Tissue
Figure 3 Kidney
Collecting ducts Staining: Low Intensity: Moderate Quantity: <25% Distal tubules Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/6513_A_8_5.jpg
Normal Tissue
Figure 4 Testis
Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/6513_A_6_6.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/6513_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Enterocytes - Mucosal lymphoid cells Staining: Medium Intensity: Strong Quantity: <25% Peripheral nerve/ganglion Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/6513_A_9_3.jpg
Normal Tissue
Figure 7 Pancreas
Pancreatic endocrine cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/6513_A_1_3.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (EFO-21, SCLC-21H, U-251 MG, U-266/84, U-87 MG)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000154277-UCHL1
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Specifications
- Immunogen
- Recombinant human UCHL1 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human UCHL1
- Species Reactivity
- Human
- Clone
- R37-3A-8
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- Ubiquitin C-Terminal Hydrolase L1; Ubiquitin Carboxyl-Terminal Esterase L1 (Ubiquitin Thiolesterase); Neuron Cytoplasmic Protein 9.5; Ubiquitin Thioesterase L1; Ubiquitin Thiolesterase; PGP 9.5; Uch-L1; PGP9.5; Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1; Epididymis Secretory Protein Li 53
- Gene ID
- 7345
- UniProt ID
- P09936
- Long Name
- Ubiquitin C-Terminal Hydrolase L1
- Sequence Similarities
- Belongs to the peptidase C12 family.
- Cellular Localization
- Cytoplasm, Endoplasmic reticulum, Membrane
- Post Translation Modifications
- O-glycosylated.
- Protein Refseq
- NP_004172.2
- Function
- Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Capture antibody
Isotype Control
- CAT
- Product Name
Secondary Antibody
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0615MZ | Chicken Anti-UCHL1 Polyclonal IgY | WB | Chicken antibody |
| BRD-0800MZ | Chicken Anti-PGP9.5 Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3771 | Rabbit Anti-UCHL1 Recombinant Antibody (clone DS3771AB) | WB, IP, IHC-P, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-FY1517 | Recombinant Mouse Anti-UCHL1 Antibody (clone 1B4H3) | ELISA, WB, IHC, FC | Mouse IgG2b |
| VS3-FY1518 | Recombinant Mouse Anti-UCHL1 Antibody (clone 1D1B12) | ELISA, WB, IHC, FC | Mouse IgG2b |
| VS3-FY1519 | Recombinant Mouse Anti-UCHL1 Antibody (clone 7E8-5B6-2H9) | IHC-P | Mouse IgG1 |
| VS3-FY1520 | Recombinant Rabbit Anti-UCHL1 Antibody (clone R03-6E5) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
| VS-0923-FY154 | Recombinant Mouse Anti-UCHL1 Antibody (VS-0923-FY154) | ELISA, WB | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY270 | AbPlus™ Anti-UCHL1 Magnetic Beads (JF93-08) | IP, Protein Purification | |
| VS-0724-YC1162 | AbPlus™ Anti-Uchl1 Magnetic Beads (VS-0724-YC1162) | IP, Protein Purification | |
| VS-0724-YC1164 | AbPlus™ Anti-UCHL1 Magnetic Beads (VS-0724-YC1164) | IP, Protein Purification | |
| VS-0724-YC1237 | AbPlus™ Anti-Uchl1 Magnetic Beads (VS-0724-YC1237) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2427 | Anti-UCHL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7722 | Anti-Mouse UCHL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7723 | Anti-Rat UCHL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7721 | Anti-Human UCHL1 Immunohistochemistry Kit | IHC |
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