Recombinant Rabbit Anti-VCAM1 Antibody (VS4-CJ154)
CAT#: VS4-CJ154
This rabbit anti-VCAM1 antibody is a product that recognizes human VCAM1. The immunogen of this antibody is purified recombinant fragment of human VCAM1 expressed in E. coli. The mAb has been tested for use in ELISA, Western Blot (1:500-1:5000), Immunohistochemistry (1:50-1:500).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cell junctions.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69867/1706_F12_18_cr57d839e75f94b_selected.jpg
Normal Tissue
Figure 2 Kidney
Bowman's capsule Staining: High Intensity: Strong Quantity:>75% Distal tubules Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/154/2835_A_8_5.jpg
Normal Tissue
Figure 3 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/154/2835_A_6_6.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/154/2835_A_8_8.jpg
Normal Tissue
Figure 5 High expression in spleen
Cells in red pulp Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in white pulp Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/154/2835_B_7_4.jpg
RNA Expression
Figure 6 RNA cell line category: Group enriched (HHSteC, HSkMC)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000162692-VCAM1
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Specifications
- Immunogen
- Purified recombinant fragment of human VCAM1 expressed in E. coli
- Host Species
- Rabbit
- Type
- Rabbit IgG1
- Specificity
- Human VCAM1
- Species Reactivity
- Human
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 150mM NaCl, PBS, pH 7.4.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Western Blot (1:500-1:5000), Immunohistochemistry (1:50-1:500).
Target
- Alternative Names
- CD106; INCAM-100
- Gene ID
- 7412
- UniProt ID
- P19320
- Long Name
- Vascular cell adhesion molecule 1
- Cellular Localization
- Membrane
- Post Translation Modifications
- Sialoglycoprotein.
- Protein Refseq
- NP_001069.1; NP_001186763.1; NP_542413.1
- Function
- Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "VCAM1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1467z | Mouse Anti-VCAM1 Recombinant Antibody (clone 23B9) | ELISA, FC, IHC, FuncS | Mouse IgG1 |
| MOB-2174MZ | Recombinant Mouse Anti-Human VCAM1 Antibody (clone TUB) | ELISA, FC, IHC-Fr, IP | Mouse antibody |
| HPAB-AP205-YC | Human Anti-VCAM1 Recombinant Antibody (HPAB-AP205-YC) | IHC, DB | Human IgG |
| HPAB-AP206-YC | Human Anti-VCAM1 Recombinant Antibody (HPAB-AP206-YC) | IHC, DB | Human IgG |
| HPAB-AP207-YC | Human Anti-VCAM1 Recombinant Antibody (HPAB-AP207-YC) | IHC, DB | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-061 | Recombinant Anti-Human VCAM1 VHH Single Domain Antibody | WB, ELISA, IP, FuncS | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B067(A) | Recombinant Anti-human VCAM-1 Intrabody [(D-Arg)9] | FACS, ELISA, IF, FuncS | scFv-(D-Arg)9 |
| IAB-B067(G) | Recombinant Anti-human VCAM-1 Intrabody [+36 GFP] | FACS, ICC, FuncS | scFv-(+36GFP) |
| IAB-B067(T) | Recombinant Anti-human VCAM-1 Intrabody [Tat] | PCA, WB, FuncS | scFv-Tat |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-249CQ | Anti-Human VCAM1 Recombinant Antibody (H6) | ELISA | Human antibody |
| TAB-249CQ-S(P) | Anti-Human VCAM1 Recombinant Antibody scFv Fragment (H6) | ELISA, FC, Neut | Human antibody |
| TAB-249CQ-F(E) | Anti-Human VCAM1 Recombinant Antibody Fab Fragment (H6) | ELISA, FC, Neut | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-250CQ | Human Anti-VCAM1 Recombinant Antibody (TAB-250CQ) | ELISA, FC, WB, IHC | Humanized IgG |
| TAB-251CQ | Human Anti-VCAM1 Recombinant Antibody (TAB-251CQ) | ELISA, FC, WB, IHC | Humanized IgG |
| TAB-250CQ-S(P) | Human Anti-VCAM1 Recombinant Antibody; scFv Fragment (TAB-250CQ-S(P)) | ELISA, FC, WB, IHC | Humanized scFv |
| TAB-250CQ-F(E) | Human Anti-VCAM1 Recombinant Antibody; Fab Fragment (TAB-250CQ-F(E)) | ELISA, FC, WB, IHC | Humanized Fab |
| TAB-251CQ-F(E) | Human Anti-VCAM1 Recombinant Antibody; Fab Fragment (TAB-251CQ-F(E)) | ELISA, FC, WB, IHC | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2248CQ | Rat Anti-Vcam1 Recombinant Antibody (VMC209) | FC, IP, Block, IHC | Rat IgG2a, κ |
| NEUT-2249CQ | Rat Anti-Vcam1 Recombinant Antibody (clone M/K-2.7) | Block, IF | Rat IgG1 |
| NEUT-2251CQ | Mouse Anti-VCAM1 Recombinant Antibody (clone B-K9) | Block, FuncS, FC | Mouse IgG1 |
| NEUT-2252CQ | Mouse Anti-VCAM1 Recombinant Antibody (NEUT-2252CQ) | FC, ICC, IF, Block, IP | Mouse IgG1, κ |
| NEUT-2254CQ | Mouse Anti-VCAM1 Recombinant Antibody (NEUT-2254CQ) | Block, WB, IP, ICC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2250CQ | Rat Anti-Vcam1 Recombinant Antibody (NEUT-2250CQ) | Neut, FC, IHC, IHC-Fr, IP, CyTOF | Rat IgG1 |
| NEUT-2253CQ | Mouse Anti-VCAM1 Recombinant Antibody (clone 1.G11B1) | WB, Neut, ELISA, FC, IHC, IHC-Fr, IP, ICC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3829 | Rabbit Anti-VCAM1 Recombinant Antibody (clone DS3829AB) | WB, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0218-YC-S(P) | Human Anti-VCAM1 Recombinant Antibody (clone HS-1); scFv Fragment | ELISA, Neut | Rabbit scFv |
| HPAB-0860-CN-S(P) | Human Anti-VCAM1 Recombinant Antibody (clone 7H); scFv Fragment | ELISA, Neut | Human scFv |
| HPAB-AP205-YC-S(P) | Human Anti-VCAM1 Recombinant Antibody; scFv Fragment (HPAB-AP205-YC-S(P)) | IHC, DB | Human scFv |
| HPAB-AP206-YC-S(P) | Human Anti-VCAM1 Recombinant Antibody; scFv Fragment (HPAB-AP206-YC-S(P)) | IHC, DB | Human scFv |
| FAMAB-0009JF-S(P) | Mouse Anti-VCAM1 Recombinant Antibody (clone ch2A2); scFv Fragment | FC, ELISA, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0218-YC-F(E) | Human Anti-VCAM1 Recombinant Antibody (clone HS-1); Fab Fragment | ELISA, Neut | Human Fab |
| HPAB-AP205-YC-F(E) | Human Anti-VCAM1 Recombinant Antibody; Fab Fragment (HPAB-AP205-YC-F(E)) | IHC, DB | Human Fab |
| HPAB-AP206-YC-F(E) | Human Anti-VCAM1 Recombinant Antibody; Fab Fragment (HPAB-AP206-YC-F(E)) | IHC, DB | Human Fab |
| HPAB-AP207-YC-F(E) | Human Anti-VCAM1 Recombinant Antibody; Fab Fragment (HPAB-AP207-YC-F(E)) | IHC, DB | Human Fab |
| FAMAB-0009JF-F(E) | Human Anti-VCAM1 Recombinant Antibody (clone ch2A2); Fab Fragment | FC, ELISA, FuncS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-100CN | Human Anti-VCAM1 Recombinant Antibody (clone 7C) | ELISA | Human IgG |
| NS-101CN | Human Anti-VCAM1 Recombinant Antibody (clone 4B) | ELISA | Human IgG |
| NS-100CN-F(E) | Human Anti-VCAM1 Recombinant Antibody (clone 7C); Fab Fragment | ELISA | Human Fab |
| NS-101CN-F(E) | Human Anti-VCAM1 Recombinant Antibody (clone 4B); Fab Fragment | ELISA | Human Fab |
| NS-100CN-S(P) | Human Anti-VCAM1 Recombinant Antibody (clone 7C); scFv Fragment | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY272 | AbPlus™ Anti-VCAM1 Magnetic Beads (1.G11B1) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY231 | CytoStream™ Mouse Anti-VCAM1 Recombinant Antibody (VS-0225-XY231) | FC, IHC, Block, ELISA, IP | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY210 | Human Anti-VCAM1 (clone H6) scFv-Fc Chimera | ELISA, Inhib | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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