Recombinant Rabbit Anti-VTCN1 Antibody (clone R08-7G4)

Name: Recombinant Rabbit Anti-VTCN1 Antibody (clone R08-7G4)
SKU: VS3-FY1549
Rating: 5 (1 reviews)

CAT#: VS3-FY1549

This product is a recombinant rabbit antibody that recognizes VTCN1. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence. The clone R08-7G4 is specific for human VTCN1. The antigen is human b7h4 recombinant protein.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line MCF7

Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane & cell junctions.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54200/1356_C9_3_selected.jpg

Normal Tissue Figure 2 IHC staining of human breast

Immunohistochemical staining of human breast shows moderate membranous positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54200/ihc_selected.jpg

Normal Tissue Figure 3 Colon

Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54200/147470_A_9_3.jpg

Normal Tissue Figure 4 Kidney

Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54200/147470_A_8_5.jpg

Normal Tissue Figure 5 Testis

Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54200/147470_A_4_6.jpg

Normal Tissue Figure 6 Breast

Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54200/147470_B_3_4.jpg

Normal Tissue Figure 7 Lymph node

Germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54200/147470_A_7_8.jpg

RNA Expression Figure 8 RNA cell line category: Cell line enriched (SK-BR-3)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000134258-VTCN1

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Specifications

Immunogen
Human B7H4 recombinant protein.

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human VTCN1

Species Reactivity
Human

Clone
R08-7G4

Applications
Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence

Conjugate
Unconjugated

MW
Calculated MW: 31 kDa; Observed MW: 70 kDa

Product Property

Purification
Affinity Purified

Purity
>95% as determined by SDS-PAGE

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200

Target

Alternative Names
V-Set Domain Containing T Cell Activation Inhibitor 1; Immune Costimulatory Protein B7-H4; B7 Superfamily Member 1; B7 Family Member, H4; B7 Homolog 4; B7-H4; B7h.5; B7H4; V-Set Domain-Containing T-Cell Activation Inhibitor 1

Gene ID
79679

UniProt ID
Q7Z7D3

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS3-FY1549.pdf

MSDS

MSDS - VS3-FY1549.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Secondary Antibody

CAT
Product Name

Isotype Control

CAT
Product Name

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