Recombinant Rabbit Anti-YAP1 (phospho Ser127) Antibody (clone R07-6C2)
CAT#: VS3-FY1567
This product is a recombinant rabbit antibody that recognizes YAP1. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin. The clone R07-6C2 is specific for human, mouse, rat YAP1. The antigen is a synthetic phosphopeptide corresponding to residues surrounding ser127 of human yap1.
Specifications
- Immunogen
- A synthetic phosphopeptide corresponding to residues surrounding Ser127 of human YAP1.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat YAP1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R07-6C2
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
- MW
- Calculated MW: 54 kDa; Observed MW: 70-75 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Target
- Alternative Names
- Yes Associated Protein 1; Yes-Associated Protein YAP65 Homolog; Yes-Associated Protein 1; Protein Yorkie Homolog; YAP65; Transcriptional Coactivator YAP1; Yes-Associated Protein 1, 65kDa; 5 KDa Yes-Associated Protein
- Gene ID
- 10413
- UniProt ID
- P46937
- Sequence Similarities
- Belongs to the YAP1 family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylated by LATS1 and LATS2; leading to cytoplasmic translocation and inactivation (PubMed:18158288, PubMed:20048001).
Phosphorylated by ABL1; leading to YAP1 stabilization, enhanced interaction with TP73 and recruitment onto proapoptotic genes; in response to DNA damage (PubMed:18280240).
Phosphorylation at Ser-400 and Ser-403 by CK1 is triggered by previous phosphorylation at Ser-397 by LATS proteins and leads to YAP1 ubiquitination by SCF(beta-TRCP) E3 ubiquitin ligase and subsequent degradation (PubMed:20048001).
Phosphorylated at Thr-119, Ser-138, Thr-154, Ser-367 and Thr-412 by MAPK8/JNK1 and MAPK9/JNK2, which is required for the regulation of apoptosis by YAP1 (PubMed:21364637).
Ubiquitinated by SCF(beta-TRCP) E3 ubiquitin ligase.
- Protein Refseq
- NP_001123617.1; NP_001181973.1; NP_001181974.1
- Function
- Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed:17974916, PubMed:18280240, PubMed:18579750, PubMed:21364637, PubMed:30447097).
The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed:18158288).
Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization (PubMed:25778702).
Plays a key role in controlling cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration (PubMed:18158288).
The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction (PubMed:18579750).
Suppresses ciliogenesis via acting as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1 (PubMed:25849865).
In conjunction with WWTR1, involved in the regulation of TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation (By similarity).
Isoform 2: Activates the C-terminal fragment (CTF) of ERBB4 (isoform 3).
Isoform 3: Activates the C-terminal fragment (CTF) of ERBB4 (isoform 3).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "YAP1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0305MC | Rcombinant Anti-Human YAP1 Antibody. | WB, ELISA, ICC, IF, IHC, IHC-P, S-ELISA | |
| MOB-0306MC | Rabbit Anti-Phospho-YAP (Ser127) Antibody | WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2255CT | Recombinant Mouse anti-Human YAP1 Monoclonal antibody (EML1074) | IHC-P, WB | |
| MRO-1607-CN | Rabbit Anti-YAP1 Recombinant Antibody (clone CBACN-585) | WB, IF, IHC, IP, FC | Rabbit IgG |
| VS3-CJ978 | Rabbit Anti-YAP1 Recombinant Antibody (VS3-CJ978) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| VS3-CJ979 | Rabbit Anti-YAP1 (phospho S127) Recombinant Antibody (VS3-CJ979) | WB, IHC | Rabbit IgG |
| VS3-XY1611 | Mouse Anti-YAP1 Recombinant Antibody (clone 3G1) | WB, IP, IF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3899 | Rabbit Anti-YAP1 Recombinant Antibody (clone DS3899AB) | IHC-P, WB | Rabbit IgG |
| MOR-0048-FY | Rabbit Anti-YAP1 Recombinant Antibody (clone AFY0019) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY277 | AbPlus™ Anti-YAP1 Magnetic Beads (CBACN-585) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1232 | CytoStream™ Rabbit Anti-YAP1 Recombinant Antibody (VS13-YC1232) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2503 | Anti-YAP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7974 | Anti-Mouse YAP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7973 | Anti-Human YAP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7975 | Anti-Rat YAP1 Immunohistochemistry Kit | IHC |
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