Recombinant Rabbit Anti-YWHAB Antibody (clone 6F6)

CAT#: VS4-CJ156

This rabbit anti-YWHAB antibody (clone 6F6) is a product that recognizes human, mouse, rat YWHAB. The immunogen of this antibody is a synthesized peptide derived from human YWHAB. The mAb has been tested for use in ELISA, Western Blot (1:500-1:2000), Immunofluorescence (1:50-1:200), Flow Cytometry (1:50-1:200).

Specifications

  • Immunogen
  • A synthesized peptide derived from human YWHAB
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse, Rat YWHAB
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • 6F6
  • Applications
  • ELISA, WB, IF, FC
  • Conjugate
  • Unconjugated

Product Property

  • Purification
  • Affinity chromatography
  • Purity
  • >95% as determined by SDS-PAGE
  • Format
  • Liquid
  • Buffer
  • 50% Glycerol, 150 mM NaCl, PBS, pH 7.4.
  • Preservative
  • 0.02% Sodium Azide
  • Storage
  • Store at -20°C or -80°C upon receipt. Avoid repeated freeze.

Applications

  • Application Notes
  • This antibody has been tested for use in ELISA, Western Blot (1:500-1:2000), Immunofluorescence (1:50-1:200), Flow Cytometry (1:50-1:200).

Target

  • Alternative Names
  • HS1; GW128; YWHAA; KCIP-1; HEL-S-1
  • Long Name
  • Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta
  • Sequence Similarities
  • Belongs to the 14-3-3 family.
  • Cellular Localization
  • Cytoplasm, Membrane, Vacuole
  • Post Translation Modifications
  • The alpha, brain-specific form differs from the beta form in being phosphorylated. Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.
  • Function
  • Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13.
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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

COA

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Isotype Control

Secondary Antibody

Recommended Dilution Buffer

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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