Recombinant Rabbit Anti-YWHAB Antibody (clone R08-4A7)
CAT#: VS3-FY1570
This product is a recombinant rabbit antibody that recognizes YWHAB. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation. The clone R08-4A7 is specific for human, mouse, rat YWHAB. The antigen is synthetic peptide of human 14-3-3 alpha/beta.
Tested Data
WB
Figure 1. Recombinant Rabbit Anti-YWHAB Antibody (clone R08-4A7) in WB.
Western Blot analysis of 14-3-3 α/β in rat brain, rat lung lysates using 14-3-3 α/β Antibody.
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Specifications
- Immunogen
- Synthetic peptide of human 14-3-3 alpha/beta.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat YWHAB
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R08-4A7
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 28 kDa; Observed MW: 28 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Beta; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, Alpha Polypeptide; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, Beta Polypeptide; 14-3-3 Alpha; Protein 1054; KCIP-1; Protein Kinase C Inhibitor Protein-1; Protein Kinase C Inhibitor Protein 1; Brain Protein 14-3-3, Beta Isoform
- Gene ID
- 7529
- UniProt ID
- P31946
- Sequence Similarities
- Belongs to the 14-3-3 family.
- Cellular Localization
- Cytoplasm, Membrane, Vacuole
- Post Translation Modifications
- The alpha, brain-specific form differs from the beta form in being phosphorylated. Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.
- Protein Refseq
- NP_003395.1; NP_647539.1
- Function
- Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "YWHAB"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2171MZ | Recombinant Mouse Anti-Rat YWHAB Antibody (clone 5F2) | ELISA, IP, WB | Mouse antibody |
| MRO-0001-CN | Rabbit Anti-YWHAB Recombinant Antibody (clone CBACN-001) | WB, IHC | Rabbit IgG |
| MRO-1619-CN | Rabbit Anti-YWHAB Polyclonal Antibody (MRO-1619-CN) | WB, IF, IHC | Rabbit IgG |
| VS3-CJ876 | Rabbit Anti-YWHAB Recombinant Antibody (VS3-CJ876) | WB, ICC, IHC, IP, FC | Rabbit IgG |
| VS4-CJ156 | Recombinant Rabbit Anti-YWHAB Antibody (clone 6F6) | ELISA, WB, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3904 | Rabbit Anti-YWHAB Recombinant Antibody (clone DS3904AB) | ICC, IF, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY278 | AbPlus™ Anti-YWHAB Magnetic Beads (SD0837) | IP, Protein Purification | |
| VS-0724-YC1440 | AbPlus™ Anti-YWHAB Magnetic Beads (VS-0724-YC1440) | IP, Protein Purification | |
| VS-0724-YC1491 | AbPlus™ Anti-YWHAB Magnetic Beads (VS-0724-YC1491) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2512 | Anti-YWHAB Immunohistochemistry Kit | IHC | |
| VS-0525-XY7989 | Anti-Mouse YWHAB Immunohistochemistry Kit | IHC | |
| VS-0525-XY7988 | Anti-Human YWHAB Immunohistochemistry Kit | IHC |
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