B. thuringiensis Cry1b

Bt toxin protein is a spore crystal produced by Bacillus thuringiensis in the process of forming spores, which can be divided into Cry and Cyt series 2 large families of toxic proteins, and can be used for killing insects such as lepidoptera, diptera and coleoptera. With the continuous development and maturity of transgenic technology, the environment gene has been widely used in transgenic biological control of rice, corn, cotton and other crops, and achieved relatively ideal results. However, with the application and popularization of gm crops, people have begun to pay more and more attention to the direct or indirect hazards of gm crops in terms of gene escape, changing soil microbiological ecological structure and species resistance. At present, the test boxes for the detection of Bt toxin are all ELISA detection methods based on polyclonal or monoclonal, which not only need to prepare highly purified antigens and highly specific antibodies, but also have disadvantages such as long reaction time, high impact of other factors and easy occurrence of false positives.