Studies have shown that aquaporin, as a widely existing functional transmembrane protein, plays an important role in water transport, water balance maintenance and osmotic regulation in cells under stress. As a monocotyledonous plant, it is necessary to analyze the functions of its water channel protein family members, so as to understand the mechanism of action of this gene and provide a scientific basis for alleviating the yield loss of rice under stress. Fotiadis et al. found that PIP was mainly responsible for water absorption and efflux in maintaining the integrity of cell structure, while TIP was mainly responsible for segmental bulking. Azaizeh et al. found that the water transport of barley seedlings treated with NaCl was inhibited, and the inhibition was alleviated with the addition of Ca2+.