RNF31

Enzymes, Metabolic proteins

Intracellular

Cell type enhanced (Astrocytes, Microglial cells, Oligodendrocyte precursor cells, Excitatory neurons, Plasma cells, Inhibitory neurons, Gastric mucus-secreting cells)

Low immune cell specificity

Low cell line specificity

Component of the LUBAC complex (linear ubiquitin chain assembly complex) which consists of SHARPIN, RBCK1 and RNF31 (PubMed:17006537, PubMed:21455173, PubMed:21455180, PubMed:21455181, PubMed:28481331). LUBAC has a MW of approximately 600 kDa suggesting a heteromultimeric assembly of its subunits (PubMed:17006537, PubMed:21455173, PubMed:21455180, PubMed:21455181). Associates with the TNF-R1 signaling complex (TNF-RSC) in a stimulation-dependent manner (PubMed:20005846). Interacts (via the PUB domain) with OTULIN (via the PIM motif); the interaction is direct (PubMed:23708998, PubMed:24726323, PubMed:24726327). Interacts (via the PUB domain) with VCP (via the PIM motif) (PubMed:24726327). Interacts (via the PUB domain) with SPATA2 (via the PIM motif); interaction is direct and bridges RNF31 and CYLD (PubMed:27458237, PubMed:27545878, PubMed:28189684, PubMed:27591049). Interacts with CYLD; the interaction is indirect and is mediated via SPATA2 (PubMed:27458237, PubMed:27545878, PubMed:26997266). Interacts with MUSK (By similarity). Interacts with CARD11, promoting linear ubiquitination of BCL10 (PubMed:27777308). (Microbial infection) Interacts with S. flexneri E3 ubiquitin-protein ligases IpaH1. 4 and IpaH2. 5, leading to its ubiquitination.

Transferase