XRCC6

Cancer-related genes, Plasma proteins

Intracellular

Cell type enhanced (Spermatocytes)

Low immune cell specificity

Low cell line specificity

Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70 (PubMed:11493912). Component of the core long-range non-homologous end joining (NHEJ) complex (also named DNA-PK complex) composed of PRKDC, LIG4, XRCC4, XRCC6/Ku70, XRCC5/Ku86 and NHEJ1/XLF (PubMed:12509254, PubMed:9742108, PubMed:12547193, PubMed:25941166, PubMed:25670504, PubMed:33854234). Additional component of the NHEJ complex includes PAXX (PubMed:25574025, PubMed:27601299, PubMed:27705800). Following autophosphorylation, PRKDC dissociates from DNA, leading to formation of the short-range NHEJ complex, composed of LIG4, XRCC4, XRCC6/Ku70, XRCC5/Ku86 and NHEJ1/XLF (PubMed:33854234). The XRCC5-XRCC6 dimer also associates with NAA15, and this complex binds to the osteocalcin promoter and activates osteocalcin expression (PubMed:12145306). In addition, XRCC6 interacts with the osteoblast-specific transcription factors MSX2, RUNX2 and DLX5 (PubMed:12145306). Interacts with ELF3 (PubMed:15075319). Interacts with ATP23 (PubMed:10219089). The XRCC5-XRRC6 dimer associates in a DNA-dependent manner with APEX1 (PubMed:8621488). Binds to CDK9 isoform 2 (PubMed:20493174). Identified in a complex with DEAF1 and XRCC5 (PubMed:22442688). Interacts with DEAF1 (via the SAND domain); the interaction is direct and may be inhibited by DNA-binding (PubMed:22442688). Interacts with CLU (By similarity). Interacts with NR4A3; the DNA-dependent protein kinase complex DNA-PK phosphorylates and activates NR4A3 and prevents NR4A3 ubiquitinylation and degradation (PubMed:25852083). Interacts with CYREN isoform 1 (CYREN-1) and isoform 4 (CYREN-2) (via KBM motif) (PubMed:27063109, PubMed:24610814, PubMed:28959974). Interacts (via N-terminus) with HSF1 (via N-terminus); this interaction is direct and prevents XRCC5/XRCC6 heterodimeric binding and non-homologous end joining (NHEJ) repair activities induced by ionizing radiation (IR) (PubMed:26359349). Part of the HDP-RNP complex composed of at least HEXIM1, PRKDC, XRCC5, XRCC6, paraspeckle proteins (SFPQ, NONO, PSPC1, RBM14, and MATR3) and NEAT1 RNA (PubMed:28712728). Interacts with HMBOX1 (PubMed:23685356). Interacts with ATF7 (PubMed:29490055). Interacts with APLF (via KBM motif) (PubMed:23689425, PubMed:27063109). Interacts with WRN (via KBM motif) (PubMed:27063109). The XRCC5-XRCC6 dimer associates with ALKBH2. (Microbial infection) Interacts with human T-cell leukemia virus 1/HTLV-1 protein HBZ.

Activator, DNA-binding, Helicase, Hydrolase, Lyase, Multifunctional enzyme