Does this recombinant antibody show good performance in western blot applications?

Yes, the recombinant human anti-Abeta antibody has been successfully applied in western blot experiments. Its high purity, as confirmed by RP-HPLC and SDS-PAGE, combined with its specific binding to native human antigen, ensures robust and reproducible detection in immunoblot assays.

How reliable is this antibody for functional studies?

The antibody has been extensively validated in functional studies, including ELISA, IF, and IP. Its high purity and specific recognition of Abeta provide consistent and reliable results, making it a dependable reagent in research focused on protein aggregation and signaling analysis.

Anti-Human Abeta Recombinant Antibody (TAB-229)

CAT#: TAB-229

Recombinant monoclonal antibody to Abeta. It is a monoclonal antibody for the treatment of Alzheimer's disease. A phase I clinical trial has been conducted in 2006/07. It is currently being evaluated in a prodromal Alzheimer's Disease population in the Scarlet Road study, a global phase II study of approximately 360 subjects in 100 centers in 15 countries.

Tested Data Gene Expression

ELISA

Figure 1 Anti-Human Abeta Antibody (TAB-229) in ELISA

ELISA analysis of TAB-229 was performed by coating with recombinant human Beta-amyloid 42 with His & GST Tag (10703-H20E2) (1 μg/mL). Then blocked with BSA and incubated with Anti-Human Abeta Antibody (TAB-229) at a starting concentration of 2.5 ng/mL. The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 2 Anti-Human Abeta Antibody (TAB-229) in WB

Western blot analysis was performed by loading 1 µg (lane 1, Non-Reduced) and 1 µg (lane 2, Reduced) recombinant human Beta-amyloid 42 with His & GST Tag (10703-H20E2) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least 1 hour. Membranes were probed with Anti-Human Abeta Antibody (TAB-229) at a concentration of 1 μg/mL overnight at 4°C on a rocking platform. Then probed with Goat anti-Human IgG HRP secondary antibody at a dilution of 1:8,000 for one hour. Chemiluminescent was detected.

Figure 3 Anti-Human Abeta Antibody in DB

Dot Blot analysis of TAB-229 was performed by coating with Human Abeta Protein (His Tag).

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Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus & vesicles.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1462/if_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1462/60_E2_1_blue_red_green.jpg

Subcellular Location Figure 3 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1462/61_E2_1_blue_red_green.jpg

Normal Tissue Figure 4 Cerebral cortex

Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/157/2073_B_7_5.jpg

RNA Expression Figure 5 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000142192-APP

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Specifications

Immunogen
The details of the immunogen for this antibody are not available.

Host Species
Human

Derivation
Human

Type
IgG1 - kappa

Specificity
Tested positive against native human antigen.

Species Reactivity
Human

Applications
FuncS, IF, Neut, ELISA, FC, IP, ICC, IHC, Inhib

MW
146.3 kDa

Related Disease
Alzheimer's disease (AD)

Product Property

Purity
>95.0%, determined by analysis by RP-HPLC & analysis by SDS-PAGE.

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
The APP antibody has been reported in applications of FuncS, IF, Neut, ELISA, FC, IP, ICC, IHC, Inhib.

Target

Alternative Names
APP; amyloid beta (A4) precursor protein; AD1, Alzheimer disease; amyloid beta A4 protein; peptidase nexin II; preA4; protease nexin-II; peptidase nexin-II; beta-amyloid peptide; alzheimer disease

Gene ID
351

UniProt ID
P05067

REVIEWS AND Q&AS CITATIONS RESOURCES DOWNLOADS RELATED PRODUCTS

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Customer Review

There are currently no Customer reviews or questions for TAB-229. Click the button above to contact us or submit your feedback about this product.

Breast cancer biomarkers at key points during disease progression

Robust in WB

I have applied this human recombinant antibody against Abeta in western blot and ELISA studies. The high level of purity and the excellent binding specificity to native human antigen have been very useful in detecting Abeta. It has contributed to clear and reproducible band results in my experiments.

Impressive Specificity

The recombinant antibody to Abeta shows impressive specificity and strong signals in multiple immunoassays. I appreciate its validated performance (by RP-HPLC and SDS-PAGE analysis) and find it very suitable for my research investigating APP processing and signal transduction related to Abeta detection.

Q&As

Does this recombinant antibody show good performance in western blot applications?

A: Yes, the recombinant human anti-Abeta antibody has been successfully applied in western blot experiments. Its high purity, as confirmed by RP-HPLC and SDS-PAGE, combined with its specific binding to native human antigen, ensures robust and reproducible detection in immunoblot assays.
How reliable is this antibody for functional studies?

A: The antibody has been extensively validated in functional studies, including ELISA, IF, and IP. Its high purity and specific recognition of Abeta provide consistent and reliable results, making it a dependable reagent in research focused on protein aggregation and signaling analysis.

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-229, RRID: AB_3111888)

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Biosimilar Overview

Please refer to Gantenerumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Gantenerumab.

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-229.pdf

MSDS

MSDS - TAB-229.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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MOR-0082-FY	Rabbit Anti-APP Recombinant Antibody (clone AFY0053)	ICC, IHC-P, WB	Rabbit IgG

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