Recombinant Human Antibody (CT149) is capable of binding to IAV HA, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-IAV HA mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-IAV HA mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This antibody is found to neutralize all tested group 2 and some group 1 influenza A viruses by inhibiting low pH-induced, HA-mediated membrane fusion.
Figure 1 Recombinant Human Anti-IAV HA Antibody (PABL-213) in SDS-PAGE
SDS-PAGE analysis of PABL-213 in non-reduced (Lane 1, 2 μg) and β-mercaptoethanol-reduced (Lane 2, 2 μg) conditions.
Figure 2 Recombinant Human Anti-IAV HA Antibody (PABL-213) in HPLC
The purity of PABL-213 was greater than 95% as determined by SEC-HPLC.
Figure 3 Anti-IAV HA Recombinant Antibody (PABL-213) in ELISA
ELISA analysis of PABL-213 was performed by coating with Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin / HA Protein (His Tag). Then blocked with BSA and incubated with PABL-213. The HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 4 Anti-IAV HA Recombinant Antibody (PABL-213) in ELISA
ELISA analysis of PABL-213 was performed by coating with Influenza A H3N2 (A/Victoria/210/2009) Hemagglutinin / HA Protein (His Tag). Then blocked with BSA and incubated with PABL-213. The HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 5 Anti-IAV HA Recombinant Antibody (PABL-213) in WB
Western blot analysis of PABL-213 was performed with Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin / HA Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with PABL-213 and HRP goat anti-Human IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.8 μg)
Figure 6 Anti-IAV HA Recombinant Antibody (PABL-213) in WB
Western blot analysis of PABL-213 was performed with Influenza A H3N2 (A/Victoria/210/2009) Hemagglutinin / HA Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with PABL-213 and HRP goat anti-Human IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.8 μg)
Figure 7 Anti-IAV HA Recombinant Antibody (PABL-213) in WB
Western blot analysis of PABL-213 was performed with Influenza A H2N2 (A/Japan/305/1957) Hemagglutinin / HA Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with PABL-213 and HRP goat anti-Human IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.8 μg)
Figure 8 Anti-IAV HA Recombinant Antibody (PABL-213) in DB
Dot Blot analysis of PABL-213 was performed by coating with Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin / HA Protein (His Tag).
PABL-213 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000)
Figure 9 Anti-IAV HA Recombinant Antibody (PABL-213) in DB
Dot Blot analysis of PABL-213 was performed by coating with Influenza A H3N2 (A/Victoria/210/2009) Hemagglutinin / HA Protein (His Tag).
PABL-213 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000)
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
PABL-482 | Recombinant Human Anti-H1N1 HA Antibody (CH65) | FuncS, Neut, WB | IgG |
PABL-483 | Recombinant Human Anti-H1N1 HA Antibody (CH67) | IF, FC | IgG |
PABL-484 | Recombinant Human Anti-H1N1 HA Antibody (I2) | Neut | IgG |
PABL-485 | Recombinant Mouse Anti-H1N1 HA Antibody | WB, ELISA, FuncS | IgG |
PABL-487 | Recombinant Human Anti-H2N2 HA Antibody (2G1 ) | FuncS | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBZ-076 | Recombinant Mouse Anti-IAV HA1 Antibody Fab Fragment (26/9) | ELISA | IgG |
PFBC-078 | Recombinant Mouse Anti-IAV HA (T131I) Antibody Fab Fragment | Neut | IgG |
PFBZ-078 | Recombinant Mouse Anti-IAV HA heptapeptide (DVPDYAS) Antibody Fab Fragment (17/9) | ELISA | IgG |
PFBL-479 | Recombinant Human Anti-H1N1 HA Antibody Fab Fragment (CH65) | WB, ELISA, FuncS | Fab |
PFBL-480 | Recombinant Human Anti-H1N1 HA Antibody Fab Fragment (CH67) | Neut | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBC-160 | Human Anti-HA Recombinant Antibody; Fab Fragment (PFBC-160) | Neut, ELISA, FC | Human Fab |
FAMAB-0254-YC-S(P) | Recombinant Mouse Anti-HA Antibody scFv Fragment (H153-70A12) | ELISA | Mouse scFv |
FAMAB-0277-YC-S(P) | Recombinant Mouse Anti-HA Antibody scFv Fragment (T1-107) | ELISA | Mouse scFv |
HPAB-0373-YJ-S(P) | Mouse Anti-HA Recombinant Antibody; scFv Fragment (HPAB-0373-YJ-S(P)) | ELISA, Neut, IF, FuncS | Mouse scFv |
HPAB-S0058-YC-S(P) | Mouse Anti-HA Recombinant Antibody (clone HC19); scFv Fragment | Neut | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-302CT | Anti-IAV HA Recombinant Antibody (Al7) | WB, ELISA, S-ELISA, IHC | |
MRO-303CT | Anti-IAV HA Recombinant Antibody (2242) | WB, ELISA | |
MRO-304CT | Anti-IAV HA Recombinant Antibody (10916) | WB, ELISA | |
MRO-305CT | Anti-IAV HA Recombinant Antibody (M321) | WB, ELISA | |
PABX-068-S (P) | Recombinant Mouse Anti-Hemagglutinin (HA) Antibody scFv Fragment (1F1) | WB, ELISA, FuncS | scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-265CT-F(E) | Anti-IAV HA Recombinant Antibody Fab Fragment (D4-4) | WB, FC, IP | Human antibody |
MRO-266CT-F(E) | Anti-IAV HA Recombinant Antibody Fab Fragment (D4-5) | WB, FC, IP | Human antibody |
MRO-267CT-F(E) | Anti-IAV HA Recombinant Antibody Fab Fragment (D5-3) | WB, FC, IP | Human antibody |
MRO-268CT-F(E) | Anti-IAV HA Recombinant Antibody Fab Fragment (D6-1) | WB, FC, IP | Human antibody |
MRO-269CT-F(E) | Anti-IAV HA Recombinant Antibody Fab Fragment (IgA 59) | WB, Neut | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-070-S (P) | Recombinant Mouse Anti-HA1 Antibody scFv Fragment (Fab17/9) | WB, ELISA, Neut, FuncS | scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-4209 | Hi-Affi™ Recombinant Rabbit Anti-HA Monoclonal Antibody (SI287DS) | ELISA, ICC/IF, IF, FC | IgG |
MOR-4210 | Hi-Affi™ Recombinant Rabbit Anti-HA Monoclonal Antibody (SI288DS) | ELISA, ICC/IF, IF, FC | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MHC-CN0765 | PE-DRB1*04:01/Influenza HA (PKYVKQNTLKLAT) MHC Tetramer | FCM, IHC | |
MHC-CN0766 | APC-DRB1*04:01/Influenza HA (PKYVKQNTLKLAT) MHC Tetramer | FCM, IHC | |
MHC-CN0767 | FITC-DRB1*04:01/Influenza HA (PKYVKQNTLKLAT) MHC Tetramer | FCM, IHC | |
MHC-YF182 | DRB1*01:01/Influenza HA (PKYVKQNTLKLAT) MHC Monomer | MHC Multimer | |
MHC-YF183 | DRB1*04:01/Influenza HA (PKYVKQNTLKLAT) MHC Monomer | MHC Multimer |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-1169-FY | Llama Anti-HA Recombinant Single Domain Antibody (HPAB-1169-FY) | Neut | Llama VHH |
HPAB-1170-FY | Llama Anti-HA Recombinant Single Domain Antibody (HPAB-1170-FY) | Neut | Llama VHH |
HPAB-1171-FY | Llama Anti-HA Recombinant Single Domain Antibody (HPAB-1171-FY) | Neut | Llama VHH |
HPAB-1172-FY | Llama Anti-HA Recombinant Single Domain Antibody (HPAB-1172-FY) | Neut | Llama VHH |
HPAB-1173-FY | Llama Anti-HA Recombinant Single Domain Antibody (HPAB-1173-FY) | Neut | Llama VHH |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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