Human Anti-IFNG Recombinant Antibody (clone AF-2) (CAT#: PABW-079)

Recombinant Humanized Antibody (AF-2) is capable of binding to IFNG, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-IFNG mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti- IFNG mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. The AF2 antibody prevented the up regulation to a greater extent than any other member of the panel.

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Figure 1 Effect of AF-2, a neutralizing Ab to human IFN-γ, on PMA-induced E-selectin expression on HUVEC, pretreated with IFN-γ.

Figure 1 Effect of AF-2, a neutralizing Ab to human IFN-γ, on PMA-induced E-selectin expression on HUVEC, pretreated with IFN-γ.

HUVEC were cultured with 200 ng/ml IFN-γ ▪ or medium alone □ for 24 h in the presence or absence of the indicated Abs at 10 μg/ml. PMA, 1 ng/ml, was then added for an additional 4 h, and cells were analyzed for expression of E-selectin by a cell-based ELISA, as described in Materials and Methods. The relative amount of E-selectin measured is indicated by the OD at 450 nm. The mean ± SD from triplicate samples in a single, representative experiment is shown. **Indicates p < 0.005; *indicates p < 0.05; NS indicates not significant, comparing with and without IFN-γ.

Melrose, J. , Tsurushita, N. , Liu, G. , & Berg, E. L. . (1998). Ifn-γ inhibits activation-induced expression of e- and p-selectin on endothelial cells. Journal of Immunology, 161(5), 2457-64.


Specifications

  • Immunogen
  • Human interferon, gamma
  • Host Species
  • Human
  • Derivation
  • Humanized
  • Type
  • Humanized IgG
  • Specificity
  • Human IFNG
  • Species Reactivity
  • Human
  • Clone
  • AF-2
  • Applications
  • Neut, ELISA

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The IFNG antibody has been reported in applications of Neutralization. AF-2 (murine IgG2b), a neutralizing mAb to human IFN-γ.

Target

  • Alternative Names
  • IFNG; interferon, gamma; IFG; IFI; interferon gamma; IFN-gamma; immune interferon;

Related Resources

  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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