The recombinant scFv fragment was derived from mAb 926. The antibody fragment specifically binds to human IL-6 and can be potentially used in the treatment studies of inflammatory diseases and autoimmune diseases.
Figure 1 shows the results of an IL-6 binding inhibition assay in which human recombinant IL-6 was incubated with various anti-IL-6 antibodies, followed by the addition of IL-6 receptor-bearing U266 cells.
A purified mouse IL-6 antibody and an APC-conjugated polyclonal goat-anti-mouse secondary antibody were then added. Flow cytometry analysis was used to quantitate the IL-6 blocking activity at various anti-IL-6 antibody concentrations.
Figure 2 show the results of experiments in which IL-6-specific mAbs were tested for their ability to inhibit the interaction between IL-6 and the IL-6 receptor expressed on U266 cells, as analyzed by flow cytometry.
The concentration of mAb was 0.5 μg/ml. For each mAb, the four columns from left to right indicate the percent inhibition of IL-6 binding to IL-6 receptor at IL-6 concentrations of 100.0, 50.0, 25.0, and 12.5 ng/ml, respectively.
Figure 3 is a summary chart showing the results of three separate IL-6 binding inhibition assays.
Figure 4 shows the results of experiments in which IL-6-specific mAbs were tested for their ability to inhibit the IL-6-dependent proliferation of the human U266 myleoma cell line.
For each mAb, the six columns from left to right indicate the percent inhibition of IL-6 (500 pg/ml)-induced U266 cell proliferation at mAb concentrations of 1000, 333, 111, 37, 12, and 4 ng/ml, respectively.
Figure 5 shows the results of experiments in which IL-6-specific mAbs were tested for their ability to detect natural intracytoplasmic IL-6 in human monocytes from peripheral blood mononuclear cells (PBMNC) activated for 24 hours with LPS.
The thicker lines represent mAb detection of IL-6 from LPS-stimulated monocytes while the thinner lines represent absence of added antibody.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
TAB-097 | Anti-Human IL6 Recombinant Antibody (Sirukumab) | FC, IP, ELISA, Neut, FuncS, IF, WB | IgG1 - kappa |
TAB-0174CL-S(P) | Human Anti-IL6 Recombinant Antibody; scFv Fragment (TAB-0174CL-S(P)) | ELISA, Block, In vivo | Human scFv |
TAB-0175CL-S(P) | Human Anti-IL6 Recombinant Antibody; scFv Fragment (TAB-0175CL-S(P)) | ELISA, Block, In vivo | Human scFv |
TAB-0176CL-S(P) | Human Anti-IL6 Recombinant Antibody; scFv Fragment (TAB-0176CL-S(P)) | ELISA, Block, In vivo | Human scFv |
TAB-680LC-S(P) | Human Anti-IL6 Recombinant Antibody; scFv Fragment (TAB-680LC-S(P)) | ELISA | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-0026CL | Human Anti-IL6 Recombinant Antibody (TAB-0026CL) | Elisa, In vivo, FuncS, Block | Chimeric (mouse/human) IgG |
TAB-0026CL-S(P) | Mouse Anti-IL6 Recombinant Antibody; scFv Fragment (TAB-0026CL-S(P)) | Elisa, In vivo, FuncS, Block | Mouse scFv |
TAB-0026CL-F(E) | Human Anti-IL6 Recombinant Antibody; Fab Fragment (TAB-0026CL-F(E)) | Elisa, In vivo, FuncS, Block | Chimeric (mouse/human) Fab |
TAB-670LC | Anti-human IL6 Recombinant Antibody (NTS) (TAB-670LC) | ELISA, Inhib | Chimeric antibody (mouse/human) |
TAB-670LC-S(P) | Anti-human IL6 scFv Fragment (NTS) | ELISA | Chimeric antibody (mouse/human) |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-0172CL | Human Anti-IL6 Recombinant Antibody (TAB-0172CL) | ELISA, Block, In vivo | Human IgG |
TAB-0172CL-F(E) | Human Anti-IL6 Recombinant Antibody; Fab Fragment (TAB-0172CL-F(E)) | ELISA, Block, In vivo | Humanized Fab |
TAB-0173CL-F(E) | Human Anti-IL6 Recombinant Antibody; Fab Fragment (TAB-0173CL-F(E)) | ELISA, Block, In vivo | Humanized Fab |
TAB-678LC | Anti-human IL6 Recombinant Antibody (BA399) (TAB-678LC) | ELISA, FC, Inhib | Humanized antibody |
TAB-678LC-F(E) | Anti-human IL6 Fab Fragment (BA399) | ELISA, FC | Humanized antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-673LC | Anti-human IL6 Recombinant Antibody | ELISA, FC | |
TAB-679LC | Anti-human IL6 Recombinant Antibody (MH166) (TAB-679LC) | ELISA, Inhib, FuncS | |
TAB-669LC-F(E) | Anti-human IL6 Fab Fragment (SK2) | ELISA | |
TAB-673LC-F(E) | Anti-human IL6 Fab Fragment | ELISA, FC | |
TAB-679LC-F(E) | Anti-human IL6 Fab Fragment (MH166) | ELISA |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-037LC | Recombinant Anti-Human IL6 Antibody (Fab glycosylation) | ELISA | Humanized antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-197LC | Recombinant Anti-Human IL6 Antibody (Non-glycosylated) | ELISA | Humanized antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
BRD-0284MZ | Chicken Anti-Interleukin-6 Polyclonal IgY | WB, Indirect ELISA | Chicken antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-1464CQ | Mouse Anti-IL6 Recombinant Antibody (clone 505E9A12A3) | Neut | Mouse IgG2a, κ |
NEUT-1465CQ | Mouse Anti-IL6 Recombinant Antibody (VMC201) | Neut, WB, IHC | Mouse IgG2 |
NEUT-1466CQ | Mouse Anti-IL6 Recombinant Antibody (clone CBL224) | IHC, ELISA, Neut | Mouse IgG1 |
NEUT-1467CQ | Mouse Anti-IL6 Recombinant Antibody (clone CBL260), FITC | FC, Neut | Mouse IgG1 |
NEUT-1468CQ | Mouse Anti-IL6 Recombinant Antibody (clone CBL414) | WB, IHC, ELISA, Neut | Mouse IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-1474CQ | Rat Anti-Il6 Recombinant Antibody (clone 32C11) | Block, ELISA, WB | Rat IgG2a |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-1814 | Rabbit Anti-IL6 Recombinant Antibody (clone DS1814AB) | WB | Rabbit IgG |
MOR-4277 | Rabbit Anti-IL6 Recombinant Antibody (clone SI360DS) | ELISA | Rabbit IgG |
MOR-4622 | Rabbit Anti-IL6 Recombinant Antibody (clone TH135DS) | WB, ELISA | Rabbit IgG |
MOR-4623 | Rabbit Anti-IL6 Recombinant Antibody (clone TH136DS) | WB | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MHC-LC3429 | PE-B8/Human IL6 (ILKQKIALD) MHC Tetramer | FCM | |
MHC-LC3430 | APC-B8/Human IL6 (ILKQKIALD) MHC Tetramer | FCM |
CAT | Product Name | Application | Type |
---|---|---|---|
NS-043CN | Human Anti-IL6 Recombinant Antibody (NS-043CN) | ELISA, WB | Human IgG1 |
NS-043CN-F(E) | Human Anti-IL6 Recombinant Antibody; Fab Fragment (NS-043CN-F(E)) | ELISA, WB | Human Fab |
NS-043CN-S(P) | Human Anti-IL6 Recombinant Antibody; scFv Fragment (NS-043CN-S(P)) | ELISA, WB | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0824-CN | Human Anti-IL6 Recombinant Antibody (clone Ab18) | ELISA | Human IgG |
HPAB-0825-CN | Human Anti-IL6 Recombinant Antibody (clone 18E) | ELISA | Human IgG1 |
HPAB-0030LY | Humanized Anti-IL6 Recombinant Antibody (HPAB-0030LY) | WB | Humanized IgG4 |
MRO-0833-CN | Mouse Anti-IL6 Recombinant Antibody (clone 1-6) | WB, IHC, IF | Mouse IgG2b |
MRO-0834-CN | Mouse Anti-IL6 Recombinant Antibody (clone 11-D4) | WB, IHC, IF, ELISA | Mouse IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
AFC-TAB-239 | Afuco™ Anti-IL6 ADCC Recombinant Antibody (Elsilimomab), ADCC Enhanced | FuncS, IF, Neut, ELISA, FC | ADCC enhanced antibody |
AFC-TAB-097 | Afuco™ Anti-IL6 ADCC Recombinant Antibody (Sirukumab), ADCC Enhanced | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
AFC-TAB-H26 | Afuco™ Anti-IL6 ADCC Recombinant Antibody (Enlimomab), ADCC Enhanced | Neut, ELISA, IF, IP, FuncS, FC | ADCC enhanced antibody |
AFC-TAB-253 | Afuco™ Anti-IL6 ADCC Recombinant Antibody (Olokizumab), ADCC Enhanced | ELISA, FC, IP, FuncS, IF | ADCC enhanced antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-1250-FY-S(P) | Mouse Anti-IL6 Recombinant Antibody; scFv Fragment (HPAB-1250-FY-S(P)) | ELISA | Mouse scFv |
HPAB-1251-FY-S(P) | Mouse Anti-IL6 Recombinant Antibody; scFv Fragment (HPAB-1251-FY-S(P)) | ELISA | Mouse scFv |
HPAB-1252-FY-S(P) | Mouse Anti-IL6 Recombinant Antibody; scFv Fragment (HPAB-1252-FY-S(P)) | ELISA | Mouse scFv |
HPAB-1253-FY-S(P) | Mouse Anti-IL6 Recombinant Antibody; scFv Fragment (HPAB-1253-FY-S(P)) | ELISA | Mouse scFv |
HPAB-1254-FY-S(P) | Mouse Anti-IL6 Recombinant Antibody; scFv Fragment (HPAB-1254-FY-S(P)) | ELISA | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-S0079-YC-S(P) | Rabbit Anti-IL6 Recombinant Antibody; scFv Fragment (HPAB-S0079-YC-S(P)) | ELISA, Block | Rabbit scFv |
HPAB-S0080-YC-S(P) | Rabbit Anti-IL6 Recombinant Antibody; scFv Fragment (HPAB-S0080-YC-S(P)) | ELISA, Block | Rabbit scFv |
HPAB-S0081-YC-S(P) | Rabbit Anti-IL6 Recombinant Antibody; scFv Fragment (HPAB-S0081-YC-S(P)) | ELISA, Block | Rabbit scFv |
HPAB-S0082-YC-S(P) | Rabbit Anti-IL6 Recombinant Antibody; scFv Fragment (HPAB-S0082-YC-S(P)) | ELISA, Block | Rabbit scFv |
HPAB-S0083-YC-S(P) | Rabbit Anti-IL6 Recombinant Antibody; scFv Fragment (HPAB-S0083-YC-S(P)) | ELISA, Block | Rabbit scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-S0166-YC | Camelid Anti-IL6 Recombinant Single Domain Antibody (HPAB-S0166-YC) | ELISA | Camelid VHH |
HPAB-S0167-YC | Camelid Anti-IL6 Recombinant Single Domain Antibody (HPAB-S0167-YC) | ELISA | Camelid VHH |
HPAB-S0168-YC | Camelid Anti-IL6 Recombinant Single Domain Antibody (HPAB-S0168-YC) | ELISA, Inhib | Camelid VHH |
HPAB-S0169-YC | Camelid Anti-IL6 Recombinant Single Domain Antibody (HPAB-S0169-YC) | ELISA, Inhib | Camelid VHH |
HPAB-S0170-YC | Camelid Anti-IL6 Recombinant Single Domain Antibody (HPAB-S0170-YC) | ELISA, Inhib | Camelid VHH |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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